Abstract: D. villosum is a rich reservoir for genie diversity of HighVlolecular Weight glutenin subunit (HMTX-GS).In this paperone Allelic Specific PCR (ASCR) marker for the amplification of the genes coding HMTXS villosum was developed on the basis of the sequences and common wheat HMW glutenin genes.PCR analysis on HMW glutenin genes of CS and its addition lines showed the specialty of this ASCR markerwhileanalysis on genes of six accessions of D. villosum showed a high degree of length polymorphism which sug-Bested that the size variation observed in different allelic subunits of D. villosum was mainly due to the different lengthof the central repetitive domain. Using this ASCR marker seven alleles at Glu V1 were found among 108 H. villosumindividuals from 18 accessions. The frequencies of those alleles were different. The allele b was found in all accessionsexcept W6 7288，and its frequency was 59.55 % of 108 individuals while that of allele d was 25. 19%.But allele a aswell as f was only found once. Therefore，different genotypes were found in the different individuals from one accessiondue to the crossollination.

Key words: Dasypyum vallosum, HMWS, Allelic specific PCR(ASCR)marker, Diversity

CLC Number: 

• S512.1