Abstract: Triplo—lethal Locus(Tpl)was surveyed as the only one that both triplo-lethal and haplo—lethal gene locus in the Drosophila genome.There Was an Osiris gene family named gene Osirisl through Osiris20 in the Tpl region.Through BLASTFP and PSI—BLAST analysis, there were only three other members of this family located outside of this locus.We called them Osiris21 to Osiris23.This gene family had the Insecta specificity.Through bioinformatics, RT-PCR and RACE methods, six silkworm genes belonging to Osiris gene family have been cloned and named as BmOsiris7, BmOsiris9, BmOsirisl8, BmOsirisl9, BmOsiris20 and BmOsiris21, respectively.Sequence analysis indicated that BmOsiris gene’S ORF were between 728 bp and 882 bp in length.The molecular size of deduced amino acid which seemed quite similar with Drosophila Osiris protein and were 26—31 kDa range.The analysis of isoeleetric point indicated that BmOsiris21 7S isoeleetric point was9.0, others were at between 6.41 and 8.66.SMART conservative region prediction on line found that this protein family belong to transmembrane protein and had specific conservative regions for the Osiris family, called DUFl676.All above dated supported the speculation that the cloned genes belonged to Osiris gene family.Phylogenetic tree analysis demonstrated paralogs of Bombyx mori and Drosophila melanogaster were much closer to the infraspeeific orthologs.Expression pattern analysis indicated that each gene in our tested samples had no tissue and space-time speciality, but expression amount had significant differences.chromosome location showed that BmOsiris7、BmOsiris9、BmOsirisl8、BmOsirisl9 and BmOsiris20 located on the Chr.26, however, BmOsiris21 individually located on Chr.4.This result Was very similar with Osiris genes from Drosophila melanogaster.

Key words: Bombyx mori, Osiris gene family, Gene cloning, Sequence analysis, Gene expression

CLC Number: 

• S881.2