Analysis of Five Genes Expression Pattern in Rice Root under Different Drought Stress

doi:10.7668/hbnxb.2010.02.010

Abstract

Abstract: Five genes expression of rice seedling roots were investigated under different concentrations of PEG treatmen.t The genes expression pattern of rice roots varied under different intensity drought stress as followed: the main changew as down-regulated under the middle and mild drought stress, the up-regu lated was the minority. The genes expression was comp lex under serious drought stress, I. e., themain change of some genes( L-ascorbate perox-i dase genes, small nuclearRNA gene Z274, form ic acid tetrahydro folate ligase genes)w as up-regulated, xy lanase inhib-i tor prote in 1precursors genew as down-regulated, themain change of acidic enchitinase genew as increase, but the up multip lew as lower than the dow nmultiple far away. Five genes expression variat ion showed six types and each type of expression was day fluctuation. Rice root systems of drough-t resistant variety had different response strategies to adapt different intensity drought stress: variety may reduce unnecessary energy and material consumption under mild and middle drought and mobilize sign ificant related substances to resist drought damage under serious drought stress.

Key words: Rea-l time PCR, Drought stress, Gene expression change, Root system

CLC Number:

S511.03

MA Ting-chen, CHEN Rong-jun, YU Rong-rong, ZENG Han-lai, ZHANG Duan-pin. Analysis of Five Genes Expression Pattern in Rice Root under Different Drought Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(2): 51-55. doi: 10.7668/hbnxb.2010.02.010.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2010.02.010

http://www.hbnxb.net/EN/Y2010/V25/I2/51

References

[1] 吴波,卢琦.我国荒漠化基本特点及加快荒漠化地区发展的意义[J].中国人口.资源与环境,2002,12(1):99-101.
[2] 王贺正,马均,李旭毅,等.水分胁迫对水稻籽粒灌浆及淀粉合成有关酶活性的影响[J].中国农业科学,2009,24(4):174-178.
[3] 杨晓玲,郭守华,杨晴,等.转BADH基因水稻幼苗抗旱性研究[J].华北农学报,2007,22(3):60-64.
[4] 李筠,邓西平,郭尚洙,等.转铜/锌超氧化物歧化酶和抗坏血酸过氧化物酶基因甘薯的耐旱性[J].植物生理与分子生物学学报,2006,32(4):451-457.
[5] 王娟,李德全.水分胁迫对玉米根系AsA-GSH循环及H2O²含量的影响[J].中国生态农业学报,2002,10(2):94-96.
[6] 陈三凤,刘德虎,李季伦.植物几丁质酶的结构、基因及其表达[J].生物工程进展,1998,18(2):33-36.
[7] 李丹丹,李春喜,邵云,等.As(Ⅲ)胁迫对小麦发芽过程中木聚糖酶活性及基因表达动态变化的影响[J].生态学报,2007,27(6):1874-1877.
[8] Li W L,Muthukrishnam S,Liu D J.Isolation of novel early salt responding genes from Wheat Triticum aestivum L.by differential display[J].Theor Appl Gene,1999,43(22):673-678.
[9] 史海水,廖祥儒,崔哲,等.植物snoRNA[J].植物生理学通讯,2004,40(6):772-774.
[10] 宗会,徐照丽,刘娥娥,等.干旱胁迫下Ca²⁺-CaM 信使系统对稻苗保护酶活性的影响[J].西北植物学报,2004,24(5):843-849.
[11] 刘志礼,李鹏云.NaCl胁迫对螺旋藻生长及抗氧化酶活性的影响[J].植物学通报,1998,3:4.
[12] Sreenivasulu N,Miranda M,Prakash H S.Transcriptome changes in foxtail millet genotypes at high salinity:Identification and characterization of a PHGPX gene specifically up-regulated by NaCl in a salt-tolerant line[J].Journal of Plant Physiolog,2004,161(4):467-477.
[13] Zhang F,Zhu L,He G.Differential gene expression in response to brown planthopper feeding in rice[J].Journal of Plant Physiology,2004,161(1):53-62.
[14] 魏敏,熊建华,李阳生,等.实时PCR定量分析干旱胁迫下水稻糖原合成酶激酶基因表达差异[J].中国水稻科学,2006,20(6):567-571.
[15] 王镜岩,朱圣庚,徐长法.生物化学[M].第3版.北京:高等教育出版社,2002.
[16] San Geeta,Garwal Anil.Grover isolation and transcription profiling of low-O² stress-associated cDNA clones from the flooding-stress-tolerant FR13A rice genotype[J].Annals of Botany,2005,96(5):831-844.
[17] Debyser W,Derdelinckx G,Delcour J A.Arabinoxylan solubilization and inhibition of the barley malt xylanolytic system by wheat during mashing with wheat wholemeal adjunct:evidence for a new class of enzyme inhibitors in wheat[J].J Am Soc Brew Chem,1997,55:153-156.
[18] Rouau X,Surget A.Evidence for the presence of a pentosanase inhibitor in wheat flours[J].J Cer Sci,1998,28:63-70.
[19] Debyser W,Peumans W J,Van Damme E M.et al.Triticum aestivum xylanase inhibitor(TAXI),a new class of enzyme inhibitor affecting breadmaking performance[J].J Cer Sci,1999,30:39-43.
[20] Lasanthi-Kudahettige,Rasika,Magneschi Leonardo,et al.Alpi amedeo and perata pierdomenico.transcript profiling of the anoxic rice coleoptile[J].Plant Physiology,2007,144:218-231.
[21] Yu J,Xiao J,Xiao R,et al.Probing gene express in live cell.One protein molecule at a time[J].Science,2006,311(5767):1600-1603.

Please choose a citation manager

Content to export