Analysis of Genetic Diversity of Capsicum Germplasm by Using SSR Markers

doi:10.7668/hbnxb.2009.S1.016

Abstract

Abstract: One hundred and nine SSR primers were used to study genetic diversity among 89 Capsicum germplasm resources that detected 231 alleles.The average number of alleles per locus was 2.66.Using NTSYS2.1.0 to cluster analysis showed that 89 Capsicum germplasm resources could be classified into 4 groups,the same species clustered together, and this indicated that it is feasible to using SSR markers to distinguish Capsicum germplasm resources ,SSR markers is an available methods to study the genetic diversity of Capsicum germplasm resources.

Key words: Capsicum, Geneticdiversity, SSRmarkers

CLC Number:

S641.03

ZHOUJing, SHENHuo-lin, YANGWen-cai, TANFang, WANGYin-lei, GUOShuang. Analysis of Genetic Diversity of Capsicum Germplasm by Using SSR Markers[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2009.S1.016.

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References

[1] 邹学校主编.中国辣椒[M].北京:中国农业出版社,2001:1-41.
[2] Wang Z,Weber J L.Surver of plant shot tandem DNA repeats[J].Theoretical and Applied Genetics,1994,88:1 -6.
[3] 艾呈祥,陆　璐,马国斌,等,两个甜瓜杂交种SSR 指纹图谱的构建[J].园艺学报,2005,32(5):902-904.
[4] Danin -Poleg Y,Reis N,Tzuri G,et al.Development andcharacterisation of microsatellite markers in Cucumis[J].Theor Appl Genet,2001,102 :61-72.
[5] 尹晗琪,刘荣云,欧阳学智,等,AFLP 法在辣椒胞质雄性不育恢复系选育中的应用[J].中山大学研究生学刊,2005,26:68 -73.
[6] 孙凌云,盖树鹏,樊治成,等,辣(甜)椒种质资源的RAPD 分析[J].西北植物学报,2005,25(5):870-875.
[7] 罗玉娣,李建国,李明芳,等,用SSR标记分析辣椒属种质资源的遗传多样性[J].生物技术通报,2006(增):337-341.
[8] 陈学军,程志芳,陈劲枫,等,辣椒种质遗传多样性的RAPD 和ISSR 及其表型数据分析[J].西北植物学报,2007,27(4):0662-0670.
[9] Paran I,JR van derVoort,Lefebvre V,et al.An integrated geneticlinkage map of pepper (Capsicum spp.)[J].Molecular-Breeding,2002,13:251-261.
[10] Wencai Yang,Xiaodong Bai,Eileen Kabelka,et al.Discoveryof single nucleotide polymorphisms in Lycopersicon esculentumby computer aided analysis of expressed sequencetags[J].Molecular Breeding,2004,14 :21-34.
[11] Yasuhiro Minamiyama,Masato Tsuro ,Masashi Hirai.An SSR-based linkage map of Capsicum annuum[J].Mol Breeding,2006,18:157-169.
[12] Oliver M,Garcia -Mas J,CardusM,et al.Construction of areference linkage map for melon[J].Genome,2001,44:836-840.
[13] Inazuka M,Tahira T,Hayashi K.One -tube post-PCR fluorescentlabeling of DNA fragments[J].Genome Res,1996 ,6:551-557.
[14] 韩占兵,李婉平.肉鸽品系的随机扩增多态性DNA(RAPD)分析[J].西北农林科技大学学报,2003,12 :113-116.
[15] Lefebvre V,Palloix A,RivesM.Nuclear RFLP between peppercultivars (Capsicum annuum L)[J].Euphytica,1993 ,71:112 -121.
[16] Lcaiza -Figueroa F K,Ritland J A,Laborde.C,et al.Patternof genetic variation of the genus Capsicum(Solanaceae)in Mexico[J].Pl Syst Evol,1989,165:159-188.
[17] 王　玲,郑金贵,赖钟雄,等,辣椒遗传多样性的RAPD分析[J].福建农林大学,2003,32(2):213-216.

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