Abstract: Pepper (Capsicum annuum.) anthracnose caused by colletotrichum capsici is the most destructive disease reducing marketable fruit yields and decreasing fruit quality of pepper in China. This experiment as a fundamental research for resistance breeding was conducted to establish more reliable screening method based on sporulation of colletotrichum capsici for inoculum preparation, and inoculation methods combining inoculation stage, inoculation method, inoculum concentrations, post-inoculation wetness duration and evaluation date for testing resistance. Sporulation of colletotrichum capsici was most abundant on Cowpea pod tissue medium for 10-14 days under 28 ℃ with darkness. A 0.02 mL droplet of irroculum(3×106 spores/mL)was placed on the wound-ripe red fruits pricked with multi-pins, and keep humidity of 90%-100% at (26±2) ℃ for 3 days with darkness, the 7th day after inoculation was the optimal period for evaluation resistance in pepper. With this screening method,7 resistant varieties (lines) was obtained from 19 pepper germplasm, which can be used to pepper breeding of disease resistance.

Key words: Pepper(Capsicum annuum), Anthracnose, Identification of disease resistance, Application

CLC Number: 

• S436.418