Effects of Nitrogen Application on Yield and Physiology of Late Rice under Drought Condition

doi:10.7668/hbnxb.20191791

Abstract

Abstract: In order to explore the effect of nitrogen management on double cropping super rice late rice under drought conditions.Wufengyou T025, a super hybrid late rice variety, was selected. Under the normal or drought conditions at the young panicle differentiation stage, the yield and related physiological and ecological indexes were investigated by applying more nitrogen tiller fertilizer (before drought) and nitrogen panicle fertilizer (after drought).The results showed that:Drought lead to a significant decrease in rice yield, and the young panicle differentiation stage was the sensitive stage of water deficit in late rice. Under the condition of normal water or drought conditions, the yield of higher tiller fertilizer application was significantly higher than that of higher panicle fertilizer application, and the tillering stage was the key period for nitrogen requirement of late rice. The effective panicles per plant and seed setting rate of higher tiller fertilizer application were significantly higher than those of higher panicle fertilizer application. Compared with the application of higher nitrogen tiller fertilizer, the photosynthetic rate (Pn), transpiration rate (Tr) and other photosynthetic indexes of higher panicle fertilizer application were increased in different degrees at heading and flowering stages, but the nitrogen and dry matter transport rate of stem and leaf to panicle was low. The retention was serious, and the nitrogen transported to the panicle was relatively low. The contents of osmotic adjustment substances and the malondialdehyde (MDA) content in leaves decreased in the late growth stage, and the activities of nitratase (NR) and glutamic-pyruvic transaminase(GPT) increased in the short term after drought for higher tiller fertilizer application.Therefore, there is a certain effect of "regulating water with nitrogen" in rice. Properly increasing the ratio of nitrogen fertilizer at tillering stage can reduce the yield loss of late rice caused by drought stress at young panicle differentiation stage.

Key words: Rice, Nitrogen application, Drought, Yield, Physiology

CLC Number:

HUANG Huixian, SHEN Tianhua, ZHONG Lei, DU Jie, FU Junru, ZHOU Dahu, HE Haohua, CHEN Xiaorong. Effects of Nitrogen Application on Yield and Physiology of Late Rice under Drought Condition[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(3): 150-158. doi: 10.7668/hbnxb.20191791.

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References

[1] Wang W X,Li Y Y,Dang P Q,Zhao S J,Lai D W,Zhou L G.Rice secondary metabolites:structures,roles,biosynthesis,and metabolic regulation[J]. Molecules,2018,23(12):3098.doi:10.3390/molecules23123098.
[2] Zhang S G,Shen T L,Yang Y C,Li Y C,Wan Y S,Zhang M,Tang Y F,Allen S C. Controlled-release urea reduced nitrogen leaching and improved nitrogen use efficiency and yield of direct-seeded rice[J]. Journal of Environmental Management,2018,220:191-197.doi:10.1016/j.jenvman.2018.05.010.
[3] 徐国伟,孙会忠,陆大克,王贺正,李友军. 不同水氮条件下水稻根系超微结构及根系活力差异[J]. 植物营养与肥料学报,2017,23(3):811-820.doi:10.11674/zwyf.16291.
Xu G W,Sun H Z,Lu D K,Wang H Z,Li Y J. Differences in ultrastructure and activity of rice roots under different irrigation and nitrogen supply levels[J]. Journal of Plant Nutrition and Fertilizers,2017,23(3):811-820.
[4] Elazab A,Serret M D,Araus J L. Interactive effect of water and nitrogen regimes on plant growth,root traits and water status of old and modern durum wheat genotypes[J]. Planta,2016,244(1):125-144.doi:10.1007/s00425-016-2500-z.
[5] 马守臣,张绪成,段爱旺,杨慎骄,孙景生. 施肥对冬小麦的水分调亏灌溉效应的影响[J]. 农业工程学报,2012,28(6):139-143.doi:10.3969/j.issn.1002-6819.2012.06.023.
Ma S C,Zhang X C,Duan A W,Yang S J,Sun J S. Regulated deficit irrigation effect of winter wheat under different fertilization treatments[J]. Transactions of the Chinese Society of Agricultural Engineering,2012,28(6):139-143.
[6] 段宏波,汪寿阳. 减缓与适应:中国应对气候变化的成本收益分析[J]. 中国科学院院刊,2018,33(3):284-290.doi:10.16418/j.issn.1000-3045.2018.03.008.
Duan H B,Wang S Y. Mitigation and adaptation:Cost-benefit analysis on copping with climate change in China[J]. Bulletin of Chinese Academy of Sciences,2018,33(3):284-290.
[7] Abhinandan K,Skori L,Stanic M,Hickerson N M N,Jamshed M,Samuel M A. Abiotic stress signaling in wheat-an inclusive overview of hormonal interactions during abiotic stress responses in wheat[J]. Frontiers in Plant Science,2018,9:734.doi:10.3389/fpls.2018.00734.
[8] 杨瑰丽,杨美娜,黄翠红,曲志恒,陈志强,王加峰. 水稻幼穗分化期的抗旱性研究与综合评价[J]. 华北农学报,2015,30(6):140-145.doi:10.7668/hbnxb.2015.06.021.
Yang G L,Yang M N,Huang C H,Qu Z H,Chen Z Q,Wang J F. Comprehensive evaluation of drought resistance during rice panicle differentiation stage[J]. Acta Agriculturae Boreali-Sinica,2015,30(6):140-145.
[9] 夏琼梅,毛桂祥,王定开,王月英,李贵勇,邓安凤,龙瑞平,杨从党. 幼穗分化期至齐穗期水分胁迫对水稻产量及功能叶性状的影响[J]. 干旱地区农业研究,2015,33(3):111-116.doi:10.7606/j.issn.1000-7601.2015.03.18.
Xia Q M,Mao G X,Wang D K,Wang Y Y,Li G Y,Deng A F,Long R P,Yang C D. Effects of water stress on rice grain yield and functional leaf traits during panicle differentiation to full heading stages[J]. Agricultural Research in the Arid Areas,2015,33(3):111-116.
[10] Anupama A,Bhugra S,Lall B,Chaudhury S,Chugh A. Assessing the correlation of genotypic and phenotypic responses of indica rice varieties under drought stress[J]. Plant Physiology and Biochemistry,2018,127:343-354.doi:10.1016/j.plaphy.2018.04.001.
[11] Mastrodomenico A T,Purcell L C,King A C. The response and recovery of nitrogen fixation activity in soybean to water deficit at different reproductive developmental stages[J]. Environmental and Experimental Botany,2013,85:16-21.doi:10.1016/j.envexpbot.2012.07.006.
[12] 胡语妍,万文亮,王江丽,刁明. 不同水氮处理对滴灌春小麦氮素积累转运及产量的影响[J].石河子大学学报(自然科学版),2018,36(4):448-456.doi:10.13880/j.cnki.65-1174/n.2018.04.009.
Hu Y Y,Wan W L,Wang J L,Diao M. Effects of different water and nitrogen application rates on the accumulation and translocation of nitrogen and yield of spring wheat under drip irrigation[J]. Journal of Shihezi University(Natural Science),2018,36(4):448-456.
[13] 谷晓博,李援农,杜娅丹,吴国军,周昌明,任全茂,杨丹. 不同施氮水平对返青期水分胁迫下冬油菜补偿效应的影响[J]. 中国生态农业学报,2016,24(5):572-581.doi:10.13930/j.cnki.cjea.150995.
Gu X B,Li Y N,Du Y D,Wu G J,Zhou C M,Ren Q M,Yang D. Compensative impact of winter oilseed rape (Brassica napus L.) affected by water stress at re-greening stage under different nitrogen rates[J]. Chinese Journal of Eco-Agriculture,2016,24(5):572-581.
[14] Zhong C,Jian S F,Huang J,Jin Q Y,Cao X C. Trade-off of within-leaf nitrogen allocation between photosynthetic nitrogen-use efficiency and water deficit stress acclimation in rice (Oryza sativa L.)[J]. Plant Physiology and Biochemistry,2019,135:41-50.doi:10.1016/j.plaphy.2018.11.021.
[15] Martínez-Alcántara B,Jover S,Qui n ones A,Forner-Giner M,ÁRodríguez-Gamir J,Legaz F,Primo-Millo E,Iglesias D J. Flooding affects uptake and distribution of carbon and nitrogen in citrus seedlings[J]. Journal of Plant Physiology,2012,169(12):1150-1157.doi:10.1016/j.jplph.2012.03.016.
[16] 熊强强,钟蕾,陈小荣,朱昌兰,彭小松,贺晓鹏,傅军如,边建民,胡丽芳.穗分化期旱涝急转对双季超级杂交稻叶片稳定性δ¹³C和δ¹⁵N同位素比值的影响[J].核农学报,2017,31(3):559-565.doi:10.11869/j.issn.100-8551.2017.03.0559.
Xiong Q Q,Zhong L,Chen X R,Zhu C L,Peng X S,He X P,Fu J R,Bian J M Á,Hu L F. Effects of drought-floods abrupt alternation during panicle initiation stage on δ¹³C and δ¹⁵N stable isotope ratios of leaves in double-season super hybrid rice[J]. Journal of Nuclear Agricultural Sciences,2017,31(3):559-565.
[17] Campion E M,Loughran S T,Walls D. Protein quantitation and analysis of purity[J]. Methods in Molecular Biology,2017,1485:225-255.doi:10.1007/978-1-4939-6412-3_12.
[18] Abid M,Ali S,Qi L K,Zahoor R,Tian Z W,Jiang D,Snider J L,Dai T B. Physiological and biochemical changes during drought and recovery periods at tillering and jointing stages in wheat (Triticum aestivum L.)[J]. Scientific Reports,2018,8(1):4615.doi:10.1038/s41598-018-21441-7.
[19] Ohgami N,Upadhyay S,Kabata A,Morimoto K,Kusakabe H,Suzuki H. Determination of the activities of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase in a microfluidic system[J]. Biosensors and Bioelectronics,2007,22(7):1330-1336.doi:10.1016/j.bios.2006.06.007.
[20] Fedorova K,Kayumov A,Woyda K,Ilinskaja O,Forchhammer K. Transcription factor TnrA inhibits the biosynthetic activity of glutamine synthetase in Bacillus subtilis[J]. FEBS Letters,2013,587(9):1293-1298.doi:10.1016/j.febslet.2013.03.015.
[21] Sun H W,Feng F,Liu J,Zhao Q Z. Nitric oxide affects rice root growth by regulating auxin transport under nitrate supply[J]. Frontiers in Plant Science,2018,9:659.doi:10.3389/fpls.2018.00659.
[22] Mélard G. On the accuracy of statistical procedures in Microsoft Excel 2010[J]. Computational Statistics,2014,29(5):1095-1128.doi:10.1007/s00180-014-0482-5.
[23] Ding L,Gao C M,Li Y R,Li Y,Zhu Y Y,Xu G H,Shen Q R,Kaldenhoff R,Kai L,Guo S W. The enhanced drought tolerance of rice plants under ammonium is related to aquaporin (AQP)[J]. Plant Science,2015,234:14-21.doi:10.1016/j.plantsci.2015.01.016.
[24] 孙永健,孙园园,严奉君,杨志远,徐徽,李玥,王海月,马均. 氮肥后移对不同氮效率水稻花后碳氮代谢的影响[J]. 作物学报,2017,43(3):407-419.doi:10.3724/SP.J.1006.2017.00407.
Sun Y J,Sun Y Y,Yan F J,Yang Z Y,Xu H,Li Y,Wang H Y,Ma J. Effects of postponing nitrogen topdressing on post-anthesis carbon and nitrogen metabolism in rice cultivars with different nitrogen use efficiencies[J]. Acta Agronomica Sinica,2017,43(3):407-419.
[25] 王允青,郭熙盛,戴明伏. 氮肥运筹方式对杂交水稻干物质积累和产量的影响[J]. 中国土壤与肥料,2008(2):31-34.doi:10.3969/j.issn.1673-6257.2008.02.008.
Wang Y Q,Guo X S,Dai M F. Effects of nitrogen application on dry matter accumulation and yield of hybrid rice[J]. Soil and Fertilizer Sciences in China,2008(2):31-34.
[26] Singh K K,Ghosh S. Regulation of glutamine synthetase isoforms in two differentially drought-tolerant rice (Oryza sativa L.) cultivars under water deficit conditions[J]. Plant Cell Reports,2013,32(2):183-193.doi:10.1007/s00299-012-1353-6.
[27] 魏征,彭世彰,孔伟丽,高焕芝. 生育中期水分亏缺复水对水稻根冠及水肥利用效率的补偿影响[J]. 河海大学学报(自然科学版),2010,38(3):322-326.doi:10.3876/j.issn.1000-1980.2010.03.017.
Wei Z,Peng S Z,Kong W L,Gao H Z. Compensation effects of roots and shoots of rice and water and fertilizer utilization efficiency owing to rewatering of water deficit during intermediate period of bearing[J]. Journal of Hohai University (Natural Sciences),2010,38(3):322-326.
[28] 汤国平,熊强强,钟蕾,陈小荣,朱昌兰,彭小松,贺浩华. 双季早稻氮素亏缺补偿效应的形成及其生理机制初探[J]. 核农学报,2017,31(8):1585-1593.doi:10.11869/j.issn.100-8551.2017.08.1585.
Tang G P,Xiong Q Q,Zhong L,Chen X R,Zhu C L,Peng X S,He H H. Primary research on the formation and its physiological mechanism of nitrogen deficiency compensatory effects in double-season early rice[J]. Journal of Nuclear Agricultural Sciences,2017,31(8):1585-1593.
[29] Li W T,Xiong B L,Wang S W,Deng X P,Yin L N,Li H B. Regulation effects of water and nitrogen on the source-sink relationship in potato during the Tuber bulking stage[J]. PLoS One,2016,11(1):e0146877.doi:10.1371/journal.pone.0146877.
[30] Liu Z,Gao J,Gao F,Liu P,Zhao B,Zhang J. Photosynthetic characteristics and chloroplast ultrastructure of summer maize response to different nitrogen supplies[J]. Frontiers in Plant Science,2018,9:576.doi:10.3389/fpls.2018.00576.
[31] Zhou W,Yang Z P,Wang T,Fu Y,Chen Y,Hu B H,Yamagishi J,Ren W J. Environmental compensation effect and synergistic mechanism of optimized nitrogen management increasing nitrogen use efficiency in indica hybrid rice[J]. Frontiers in Plant Science,2019,10:245.doi:10.3389/fpls.2019.00245.
[32] Moles T M,Mariotti L,de Pedro L F,Guglielminetti L,Picciarelli P,Scartazza A. Drought induced changes of leaf-to-root relationships in two tomato genotypes[J]. Plant Physiology and Biochemistry,2018,128:24-31.doi:10.1016/j.plaphy.2018.05.008.
[33] 戴高兴,邓国富,周萌. 干旱胁迫对水稻生理生化的影响[J]. 广西农业科学,2006,37(1):4-6.doi:10.3969/j.issn.2095-1191.2006.01.002.
Dai G X,Deng G F,Zhou M. Effect of drought stress on physiology and biochemistry of rice[J]. Guangxi Agricultural Sciences,2006,37(1):4-6.
[34] Agurla S,Gahir S,Munemasa S,Murata Y,Raghavendra A S. Mechanism of stomatal closure in plants exposed to drought and cold stress[J]. Survival Stragtegies in Extreme Cold and Desiccation,2018,1081:215-232.doi:10.1007/978-981-13-1244-1_12.
[35] Reguera M,Peleg Z,Abdel-Tawab Y M,Tumimbang E B,Delatorre C A,Blumwald E. Stress-induced cytokinin synthesis increases drought tolerance through the coordinated regulation of carbon and nitrogen assimilation in rice[J]. Plant Physiology,2013,163(4):1609-1622.doi:10.1104/pp.113.227702.
[36] Khammari I,Galavi M,Ghanbari A,Solouki M,Asgharipour M R,Poorchaman A. The effect of drought stress and nitrogen levels on antioxidant enzymes,proline and yield of Indian senna (Cassia angustifolia L.)[J]. Journal of Medicinal Plants Research,2012,6(11):2125-2130.doi:10.5897/JMPR11.1105.
[37] 孙永健,孙园园,李旭毅,郭翔,马均. 水氮互作下水稻氮代谢关键酶活性与氮素利用的关系[J]. 作物学报,2009,35(11):2055-2063.doi:10.3724/SP.J.1006.2009.02055.
Sun Y J,Sun Y Y,Li X Y,Guo X,Ma J. Relationship of activities of key enzymes involved in nitrogen metabolism with nitrogen utilization in rice under water-nitrogen interaction[J]. Acta Agronomica Sinica,2009,35(11):2055-2063.
[38] Goel P,Singh A K. Abiotic stresses downregulate key genes involved in nitrogen uptake and assimilation in Brassica juncea L.[J]. PLoS One,2015,10(11):e0143645.doi:10.1371/journal.pone.0143645.
[39] Nagy Z,Németh E,Guóth A,Bona L,Wodala B,Pécsváradi A. Metabolic indicators of drought stress tolerance in wheat:glutamine synthetase isoenzymes and Rubisco[J]. Plant Physiology and Biochemistry,2013,67:48-54.doi:10.1016/j.plaphy.2013.03.001.
[40] El-Khatib R T,Hamerlynck E P,Gallardo F,Kirby E G. Transgenic poplar characterized by ectopic expression of a pine cytosolic glutamine synthetase gene exhibits enhanced tolerance to water stress[J]. Tree Physiology,2004,24(7):729-736.doi:10.1093/treephys/24.7.729.
[41] Nguyen H T T,Shim I S,Kobayashi K,Usui K. Regulation of ammonium accumulation during salt stress in rice (Oryza sativa L.) seedlings[J]. Plant Production Science,2005,8(4):397-404.doi:10.1626/pps.8.397.

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