Functional Analysis of Arabidopsis AtFAO3 in Defense Against Pseudomonas syringae

doi:10.7668/hbnxb.2009.06.001

Abstract

Abstract: The long-chain alcohol oxidase(FAO) gene encodes a membrane bound,flavin2containing,hydrogen peroxide generating,long chain alcohol oxidase.There are four homologues of FAO in the Arabidopsis thaliana genome.But very little information is known about the function of Arabidopsis FAOs in response to abiotic and biotic environmental stresses.Here,we analyzed the role of the AtFAO3 from Arabidopsis in plant defense against the bacterial pathogen Pseudomonas syringae pathovar(pv.) tomato strain DC3000(Pst DC3000).AtFAO3 translational fusion with green fluorescent protein(GFP)was localized to the cell membrane in Arabidopsis protoplast cells.T2DNA insertion mutants of Atfao3 displayed less H₂O₂content in leaves under controlled conditions and accumulated less ROS levels under oxidative or biotic stress,compared to wild type (WT) plants.Responded to bacterial pathogen,Atfao3 mutant increased growth of Pst DC3000 and displayed a increased severityof disease symptoms as compared to WTplants.The Atfao3 mutant plants also exhibited reduced expression of the defense related PR1,PRand PAL genes after pathogen infection.On the basis of analysis of T2DNA insertion mutants,we confirmed that AtFAO3 played a key role in defense against bacterial pathogens.

Key words: AtFAO3, T2DNA mutant, Plant defense, ROS

CLC Number:

S432.2

SUN Feng, YANG Xue, WANG Xiao-li, DONG Han-song. Functional Analysis of Arabidopsis AtFAO3 in Defense Against Pseudomonas syringae[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(6): 1-5. doi: 10.7668/hbnxb.2009.06.001.

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