In Silico Cloning and Expression Analysis of Glycine Max Pyridoxal Kinase Gene

doi:10.7668/hbnxb.2009.03.005

Abstract

Abstract: Using Arabidopsis thaliana pyridoxal kinase (PK) protein sequence as a query probe to blast Glycine max EST database,a soybean PKgene cDNA of 1 102 bp was obtained and cloned by RT2PCR and sequenced(GenBank Ac2 cession, DQ006813). The result confirmed that the in silico cloning cDNA sequence was accurate. It contains a complete open reading frame,from 119 bp to 1 045 bp, encodes 308 amino acids. Using semiquantitative RT2PCR,we found that PK gene was not regulated by illumination. It expressed in Glycine max leaves,stems and roots. The deduced amino acid sequence of Glycine max PKwas aligned against sequences from other species such as Solanum tuberosum, Arabidopsis thaliana, Oryza sativa, Triticum aestivum and Brassica napus. It showed the identity of 81 %,75 %,78 %,79 % and 76 %,respectively. The results revealed the Glycine max PKwas significant similarity in plants. Phylogenetic tree of plant PKs was mainly consistent with the classification system of these plants.

Key words: Glycine max, In silico cloning, Pyridoxal kinase

CLC Number:

LI Ya xuan, LI Rui, MENG Fan chen, CAI Ming hua, HU Ying kao. In Silico Cloning and Expression Analysis of Glycine Max Pyridoxal Kinase Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(3): 23-27. doi: 10.7668/hbnxb.2009.03.005.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2009.03.005

http://www.hbnxb.net/EN/Y2009/V24/I3/23

[1]	LI Shengyou, CAO Yongqiang, WANG Changling, ZHANG Lijun, WU Lishi, ZHANG Li, CHEN Yanqiu, WANG Wenbin, SONG Shuhong. Evaluation of Contribution of Different Genotypic Roots to Grain Yield in Soybean by a Grafting Experiment [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 161-166.
[2]	CUI Xiaoxia, ZHAO Shuo, GUO Shuqiao, SHU Hongmei, HE Xiaolan, NI Wanchao, GONG Yuanyong, LIU Laihua. Genome-wide Characterization and Expression Analysis of Soybean GmGLRs Gene [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(6): 1-8.
[3]	ZHANG Jinyu, CHAO Maoni, DU Hongyang, YU Deyue, HUANG Fang. Cloning and Expression Analysis of ZF-HD Transcription Factor GmZHD1 in Glycine max [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(2): 1-7.
[4]	QIN Cai-yan, HUO Jin-long, WANG Pei, WANG Shu-yan, MIAO Yong-wang, PAN Wei-rong, ZHA Xing-qin, ZENG Yang-zhi. In Silico Cloning,RT-PCR Verification and Tissues Expression of Coding Region Sequence of Novel Swine Gene KCNJ12 [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(1): 41-49.
[5]	LUO Huo-lin, LUO Li-ping, XIONG Dong-jin, YANG Bo-yun. In silico Cloning and BioinfoRmatics Analysis of GH3 in Capsicum annuum [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(6): 24-29.
[6]	ZHANG Wen-xian, JIANG Yong-mei, HE Wang-xing, GUO Wen-yan, HUANG Xiao-ju, ZHENG Su-yun. In Silico Cloning and ExpRession Analysis of 14-3-3 Gene in GanodeRma lucidum [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(5): 15-22.
[7]	LI Wen-shuang, LIU Yuan, CHANG Wen-suo, LIU Meng-xing, LI Xi-huan. In Silico Cloning and Bioinformatics Analysis of GmMDH1 Gene in Soybean( Glycine max. L) [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(3): 23-28.
[8]	ZHOU Yan-qing, TIAN Miao-miao, WANG Fang, CHEN Yan-mei, ZHANG Yong-hua, YUAN Bao-jun. Study on the Transformation of Antisense Soybean Fat Acid Desaturase to Agrobacterium tumefacien [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(2): 23-25.
[9]	HE Xiao-long, LIU Yong-bin, WANG Feng, TIAN Chun-ying, DA Lai, RONG Wei-heng,. *In Silico Cloning and Verification by RT-PCR of Differentially Expressed ADAMTS1* Gene between Mongolian Sheep Ovary** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(3): 47-51.
[10]	ZHANG Gui-rong. In Silico Cloning of Chloride Channel Gene(clc) from Zea mays and Bioinformatic Analysis [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(S2): 20-24.
[11]	ZHANG Ji-qing, CUI You-lin, DUAN Can-xing, WU Xiao-fei, WANG Xiao-ming, ZHU Zhen-dong. Identification of Pathogen Causing Soybean Charcoal Rot [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(5): 192-196.
[12]	LIU Ying, GAI Jun-yi, LU Hui-neng. Genetic Variation of Root Traits at Seedling Stage and Their Relationship with Stress Tolerances in Soybean [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(1): 114-118.
[13]	LIU Ying, GAI Jun-yi, LU Hui-neng. *Inheritance of Root Traits Related to Drought Tolerance in Soybean (Glycine max* (L) Merr.)in Huanghuaihai Area** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(5): 31-35.
[14]	MENG Xian-ping, LI Xiao-xiao, LI Ya-xuan, CAI Min-hua, HU Ying-kao. In silico Cloning of Homogentisate Phytyltransferase Gene from Soybean [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(4): 14-18.
[15]	WANG Jian-lin, QI Hua, FANG Quan-xiao, YU Gui-rui. *Diurnal Changes of Photosynthesis and Its Hysteresis to Light in Rice (Oryza sativa* L.), Soybean (Glycine max L. Merrill) and Maize (Zea mays L.)** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(2): 119-124.

Please choose a citation manager

Content to export