Cloning and Sequence Analysis of GGPS Gene in Two Sources of Tomato

doi:10.7668/hbnxb.2009.03.004

Abstract

Abstract: In this study,E6203(common tomato)and TA517(introgression lines of Solanum habrochaites)which have significant different lycopene content,were selected to be experimental materials. Specific primers had been designed, which based on GGPS fragment obtained from the subject group of fresh tomato in CAAS and the gene(DQ267903)publi2 cized in NCBI to amplify DNA and cDNA. Sequences analysis indicated that GGPS doesn′ t have intron. Putitive amino acid showed that 13 different residues are existed in the two GGPS,9 of them characters changed. The mutations lead to the difference of secondary structures,so do phosphorylation sites. It is suggested that the different lycopene content relat2 ed with amino acid mutations. 5′ 2flanking sequence were obtained by genome walking. Further analysis showed that it con2 tains significant transcriptional regulation sequences and the distribution of cis2acting element was conserved in two GGPS flanking sequence,but sequences had more difference. Semi2quantitative RT2PCR analysis presented that GGPS expressed mainly in fruit and flower.

Key words: Tomato, GGPS, 5&prime, 2flanking regions, Gene cloning, Sequence analysis

CLC Number:

Q785

ZHoNG Qiu yue, GUo Yan mei, LIANG Yan, WANG Xiaoxuan, DU Yong chen, ZHU Dewei, GAo Jian chang, DAI Shan shu. Cloning and Sequence Analysis of GGPS Gene in Two Sources of Tomato[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(3): 15-22. doi: 10.7668/hbnxb.2009.03.004.

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