In Silico Cloning and Verification by RT-PCR of Differentially Expressed ADAMTS1 Gene between Mongolian Sheep Ovary

doi:10.7668/hbnxb.2010.03.011

Abstract

Abstract: The study firstly obtained Mongolian SheePADAMTS1 gene cDNA sequences by BLAST in the Gen-Bank used differentially expressed ADAMTS1 gene fragment based on the suppression subtractive hybridization of Mongolian Sheep Ovary tissue.Based on the cDNA sequence primers were designed and cloned Mongolian SheePADAMTS1 gene by RT-PCR.The results showed that the cDNA homology between RT-PCR and in Silico Cloning is 99.4%, the sequence is 2 420 bp and included 836 bp 3' UTR and the opening reading frame( ORF) is 1 578 bp, encoding 525 amino acids; The sequence homology intercomparison with cattle,pig,human is 94%, 87%, 82%, respectively by Blastn and the amino acids homology intercomparison with cattle, pig, human is 91%, 90%, 87%, respectively by Blastp.The Mongolian SheePADAMTS1 protein have one ADAM Cysteine-Rich domain and three Thrombospondin type 1 domains by SMART program．

Key words: Mongolian sheep, ADAMTS1 Gene, In Silico Cloning, RT-PCR

CLC Number:

S826

HE Xiao-long, LIU Yong-bin, WANG Feng, TIAN Chun-ying, DA Lai, RONG Wei-heng,. In Silico Cloning and Verification by RT-PCR of Differentially Expressed ADAMTS1 Gene between Mongolian Sheep Ovary[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(3): 47-51. doi: 10.7668/hbnxb.2010.03.011.

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