Abstract: The SRAP-PCR reaction procedure and system for amplifying the watermelon genomic DNA was optimizedusing gradient experiment,so as to establish the optimum SRAP-PCR reaction conditions with high polytmrphism,good re-peatability and clear band pattern. The optimum SRAP-PCR reaction procedure was:pre-denaturation at 94 for 5 minfollowed by 5 cycles of denaturation at 94 0Cfor 1 min,anneal at 35 ?Cfor 1 min and extension at 72 ?Cfor 1 min;then 35cycles of 94 ?Cfor 1 min,50 for 1 min and 72 ?Cfor 1 min;and a final extension at 72 ?Cfor 5 min;kept at 4 ?C. Theoptimum SRAP-PCR system for a plume of 15 μL was:DNA 100 ng,Mg²⁺2. 0 mtml/L,dNTPs 0.3 mmol/ L,primer 60ng，Taq polymerase 0. 75 U. The procedure and system could meet the demands for genome SRAP amplification in C.lanatus. It was feable to apply the SRAP marker in genetic research in C.lanatus.

Key words: Watermelon, SRAP, System optimization

CLC Number: 

• S651