Establishment of cDNA-AFLP Analysis System for Cucumber

doi:10.7668/hbnxb.2007.04.027

Abstract

Abstract: After systematical studies on the factors affecting cDNA-AFLP analysis system of cucumber, an optimized system was established which showed a distinct electrophoretogram of cDNA-AFLP. The results indicated that the restriction enzyme combination was Taq I and Vsp I, the dosage of dscDNA was 300 ng, the ligation products using as template of pre-amplification were diluted to one time, and the pre-amplification products using as template of select-amplification were diluted to 30 times.

Key words: Cucumber, cDNA-AFLP, Analysis system

CLC Number:

S642.2

SUN Yong-dong, LI Zhen-xia, ZHANG Xing-guo, CHEN Bi-hua, LI Xinzheng. Establishment of cDNA-AFLP Analysis System for Cucumber[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(4): 116-119. doi: 10.7668/hbnxb.2007.04.027.

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