Somatic Tissue Culture of Hybrids Between Triticum aestivum and T. durum-Dasypyrum villosum Amphidiploid

doi:10.7668/hbnxb.1999.S1.001

Abstract

Abstract: It was easy to cross wheat with T. durum-D. villosum amphidiploid cv. TH1 and TH1W. The average percentage of seed set among 19 crosses between nine wheat varieties or bred lines selected with TH1 and TH1W was 46.7% . A total of 2316 hybrid seeds were brought about. The results of reciprocal crosses revealed that higher seed set was completed with wheat as mater-nal parent. However, the margin between reciprocal crosses were smaller in callus induction per-centage than in seed set percentage. Calli originated from immature embryos of hybrids propagated vigorously,and even differentiated plantlets directly during subculture. Two thousand and five re-generants were attained in total. The survival rate of field transplantation was higher than 95% . Observation also indicated that morphological variations were seen in few regenerats. Tissue cul-ture Had no obvious effect on improvement of fertility of T. aestivum ?T. durum-D. ?illosum amphidiploid.

Key words: Triticum aestivum T., durum-Dasypyrum villosum amphidiploid Hybridization Immature embryo culture

CLC Number:

S512.1

Li Hongjie，Shi Yunsu，Shi Zhanliang， Zhang Yanmin，Guo Beihai，Wang Zining，Wen Zhiyu. Somatic Tissue Culture of Hybrids Between Triticum aestivum and T. durum-Dasypyrum villosum Amphidiploid[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1999, 14(S1): 1-6. doi: 10.7668/hbnxb.1999.S1.001.

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