Molecular Cloning and Preliminary Analysis of Clmk-1 Gene in Cochliobolus lunatus

doi:10.3969/j.issn.1000-7091.2012.03.008

Abstract

Abstract: Cochliobolus lunatus，a fungal pathogen of foxtail millet leaf disease，affect the yield and quality when?it is epidemic. In order to understand the function of MAPK signal transduction in Cochliobolus lunatus ，a MAPK?gene was cloned by polymerase chain reaction(PCR)amplification from degenerated primer sets designed on the basis of the conserved domain of other fungal MAPK gene and the gene was called as Clmk-1. The full length cDNA?sequence of Clmk-1 in Cochliobolus lunatus was cloned by RACE methods. The Clmk-1 gene contains a 1065 bp?ORF and the deduced protein consist of 354 amino acid residues，which has the conserved TGY motif and the CD?motif. Phylogenetic analysis showed Clmk-1 gene was high similar to Hog1-type MAPK of other filamentous fungi.?The promoter region contain the binding site of transcription factor such as HSF，ADR and GCR by the bioinformatic?analysis with Promoterscan software. The chemical of U0126 inhibited the conidial germination and development of?germinating tubes. The study showed Clmk-1 was stress-activated Hog1-type MAPK and MAPK cascades regulate?the conidial germination and development of germinating tubes. The cloning of the gene will benefit the undestanding of the MAPK gene functional in Cochliobolus lunatus.

Key words: Cochliobolus lunatus, MAPK gene, Gene cloning, U0126

CLC Number:

LI Zhi-yong, HAO Zhi-min, LIU Lei,DONG Zhi-ping, DONG Jin-gao. Molecular Cloning and Preliminary Analysis of Clmk-1 Gene in Cochliobolus lunatus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(3): 41-45. doi: 10.3969/j.issn.1000-7091.2012.03.008.

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