Abstract: The viral sub-genome mRNA of Avian Reticuloendotheliosis Virus was amplified with RT-PCR. A DNA fragment was amplified which contains a part of env gene, and the size of the DNA fragment about 939 bp. The PCR products were purified and then cloned into plasmid pMD18-T, the recombinant plasmid were designated Pmd-env and analyzed by endonecleoase digestion from proper inserts. The sequence analysis of the insert fragment in the recombinant pMD-env indicated that env shared more than 94% with SNV strain, HA9901 strain, and A strain. The amino acid sequence homologywas above 94%. The env gene was subcloned into a prokaryotic expressing vector, pET-32a. Molecular cloning of env gene from REV provides a basis for the studies on protein expressing and molecular characteristic, and the constructed recombinant pET-env can be used in further protein expressing.

Key words: REV, envgene, Cloning, Prokaryotic expressing

CLC Number: 

• S432.4+1