Study on Mitochondrial DNA Diversity of the Brine Shrimp Artemia in Lagkor Co Lake of Tibet, China

doi:10.3321/j.issn:1000-7091.2005.04.020

Abstract

Abstract: The brine shrimp Artemia cysts used in this study were sampled from Lagkor Co Lake of Tibet of People's Republic of China in 1996 and 1997.It was named Artemia tibetiana.Polymorphisms of mitochondrial 12S-16S rDNA fragment were screened by using PCR-RFLP methods.The 1 500 bp fragments of mitochondrial 12S-16S rDNA were amplified on single cysts.The PCR products were digested with four restriction endonuclease recognizing four base-pair,Hpa Ⅱ,Nde Ⅱ,Taq Ⅰand Hae Ⅲ.The results showed that there were 5 haplotypes in each sample,namely,ARC1347 and ARC1348.The haplotype,AAAA was shared by both populations and had high percentage,85.0% and 83.9% respectively,in ARC1347 and ARC1348 sample.The mean genetic distance between haplotypes and the index of nucleotide diversity(π value) were 0.0350 and 0.0052 respectively,in the sample ARC1347,while the average genetic distance between haplotypes and π value were 0.0121 and 0.0013 respectively,in the sample ARC1348.There were 9 haplotypes in the two populations.The results indicated that the genetic diversity existed within the population of Artemia Tibetiana from Lagkor Co Lake of Tibet,China.

Key words: Artemia tibetiana, Cyst, Mitochondrial 12S-16S rDNA, Genetic diversity, Haplotype

CLC Number:

Q99.223+.12

WANG Xiao-mei, XIN Nai-hong, Peter Bossier. Study on Mitochondrial DNA Diversity of the Brine Shrimp Artemia in Lagkor Co Lake of Tibet, China[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2005, 20(4): 79-83. doi: 10.3321/j.issn:1000-7091.2005.04.020.

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