Abstract: To obtain transgenic potato varieties with high resistance to late blight disease from two ways of degenerating the cell walls and cell membranes by transferring both gene of rice tomautin-like protein and gene of rice chitinase 11 to virus-free potato minituber. An improved transformation system for agrobacterium mediated transformation of virus-free potato minituber was established using the tube slices as explants and optimized through the way of developing treatment method of explants, adding acetosyringone to the medium to promote the level of shooting, selecting the concentration of hormones in the shooting medium and determining the selective substances and their concentrations. Based on this system, rice thaumatin-like protein gene (TLP) and rice chitinase gene (CHI11)were transferred into virus-free potato minituber named Jinyinshu No.8 and transgenic plants which were detected by PCR and southern blot were obtained. By the greenhouse vivo inoculation and vitro inoculation using mix of outbreak and main physiological races of phytophthora infestans resistances were measured and disease developing was analyzed. It was proved that TLP and CHI11 were combined with three strains of ZGY-298,ZGY-283 and ZGY-301.Resistant experiments showed that transgenic plants significantly expressed higher resistance than control plaints and delaying the development of disease symptoms especial1y during the high peak period of disease (5-6 d after inoculation).For transgenic plants could delay the development of disease symptoms 5-6 d, It is hopeful to control the disease especially in the high peak period of disease.

Key words: Potato, Agrobacterium, Transgenic, Late-blight, Disease resistance

CLC Number: 

• S532.035.3