Primary Report on Research of Marker in Helping Gene A Transport of Melon

doi:10.3321/j.issn:1000-7091.2004.04.001

Abstract

Abstract: Segregation patterns of flower types were analyzed in F₂ and BC1 populations from each of two crosses between monoecious and andromonoecious materials of melon ( Cucumis melo). Results showed that flower type difference between the two types of parents was controlled by a pair of genes at one single locus a, with AAGG and aaGG genotypes, respectively, as flower type segregation in F₂s and BC1s from the two crosses was in agreement with simple Mendelian ratios of 3B1 and 1B1, respectively. RAPD primers were screened for polymorphisms between monoecious and andromonoecious gene pools from F₂of Mr @ YF cross using bulked seg- regant analysis ( BSA) strategy. One polymorphic fragment ( about 500 bp) was amplified, which will hopefully serve as a marker in marker-assisted selection in a backcross breeding programme for monoecious flower type.

Key words: Cucumis melo, Monoecious plant, RAPD, Genetype

CLC Number:

S652.403.6

LI Xiu-xiu, L Jing-gang, PENG Dong-xiu, ZHAO Zong-wu. Primary Report on Research of Marker in Helping Gene A Transport of Melon[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2004, 19(4): 1-3. doi: 10.3321/j.issn:1000-7091.2004.04.001.

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