Inoculating and Detecting Apple Latent Viruses of Malus xiaojinensis

doi:10.3321/j.issn:1000-7091.2003.03.018

Abstract

Abstract: Apple chlorotic leaf spot virus (ACLSV) and apple stem grooving virus (ASGV) were transmitted to Malus Xiaojinensis by in vitro micrografting. ACLSV-infected rate of M. Xiaojingensis detected by PAS-ELISA was 60.0%, and ASGV-infected rate was 73.3%. The results also indicated that virus-infected materials could be rapidly obtained by inoculated in shoots.

Key words: Micrograft in vitro, Apple latent viruses, Detect

CLC Number:

S661.1

CHENG Yu-qin, HAN Zhen-hai, XU Xue-feng. Inoculating and Detecting Apple Latent Viruses of Malus xiaojinensis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2003, 18(3): 68-70. doi: 10.3321/j.issn:1000-7091.2003.03.018.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.3321/j.issn:1000-7091.2003.03.018

http://www.hbnxb.net/EN/Y2003/V18/I3/68

References

[1] Edward M L, Cooper J I. Plant virus detecting using a new form of indirect ELISA[J]. Journal of Virological Methods, 1985, 11: 309-319.
[2] 王小凤, 李秋波, 王荣, 等. 苹果褪绿叶斑病毒和茎沟病毒的鉴定提纯和酶联法检测[J]. 微生物学报, 1992, 32(2):137-144.
[3] 洪霓,刘福昌,王国平. A蛋白酶联法检测苹果褪绿叶斑病毒和茎沟病毒的研究[J]. 中国果树, 1992, 1:44-47.
[4] Jonard R, Ludman D, Schall F, et al. Early testing of grafting incompatibities in apricot and lemon tree using in vitro techniques[J]. Sci Hort, 1982, 43: 117-128.
[5] 李嘉瑞. 苹果茎顶的显微嫁接的研究[J]. 西北农学院学报, 1985, (3): 31-37.
[6] Edna T, Shiamovitz N, Spiegel-Roy P. et al. Rapidly diagnosing grapevine corkybark by in vitro micrografting[J]. Hortscience, 1993, 28(6): 667-668.
[7] 王瑛. 小金海棠试管苗生根和移栽技术研究[D]. 北京: 中国农业大学, 1997.
[8] 张少瑜,张尊平. 落叶果树病毒病及其研究[J]. 北方果树, 2000, (4): 1-4.
[9] Kathleen H L, Rosemarie H,Janes M, et al.Monitoring prunus necrotic ringspot virus infection by hybridization with a cRNA probe after in vitro micrografting[J]. Journal of the American Society for Horticultural Science, 1995, 120(6): 928-931.
[10] Stein A, Spiegel S, Faingersh G, et al. Responses of micropropagated peach cultivars to thermotherapy for the elimination of Prunus necrotic ringspot virus[J]. Annals of Applied Biology, 1991, 119: 265-271.
[11] 曾明. 苹果属植物对苹果褪绿叶斑病毒的抗性[J].果树科学, 1994, 11(1): 5-9.
[12] Rankovic M,Sutic D. Resistance of some peach cultivars and variable pathogenicity of the sharka(plum pox)virus[J]. Acta Horticulturae, 1986,193: 193-197.
[13] Rankovic M. The degree of sensitivity of some plum cultivars and hybrids to sharka(plum pox) virus disease[J]. Acta Horticulturae, 1983, 130: 93-98.

[1]	WANG Jiarong, DONG Rui, ZHANG Mengyu, GAO Pu, ZHANG Peipei, LI Zaifeng, LIU Daqun. Identification of Leaf Rust Resistance Genes in 40 CIMMYT Wheat Lines at Seedling Stage and Adult Plant [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(2): 201-210.
[2]	JIN Fengmei, XUE Jun, SUN Haibo, HAO Zhiyu. *Molecular Detection and Partial Genomic Sequence Analysis of Tomato mosaic virus* in Tianjin Based on Small RNA Sequencing** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 176-183.
[3]	MENG Yaning, YAN Libin, ZHANG Hongxiao, ZHAO Haitao, FAN Yanqin. *Screening and Detection of Resistance to Phytophthora* Blight Gene in Pepper Using Molecular Markers** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 338-341.
[4]	CHENG Shengqun, Lü Wenhe, GAO Yanling, BAI Yanju, FAN Guoquan, ZHANG Wei, ZHANG Shu, QIU Cailing, SHEN Yu, DONG Xuezhi, BAI Yamei. *Development and Application of RT-qPCR Assay for Detection of Potato virus S* by Universal Detection System** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(6): 187-194.
[5]	JIN Fengmei, SONG Jian, XUE Jun, CHEN Dongliang, WANG Shu, ZHANG Yue, SUN Haibo. *Molecular Detection and Partial Genome Sequence Analysis of Tomato spotted wilt virus* Infected Chrysanthemum in Beijing** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(1): 175-184.
[6]	CHEN Lida, SHI Yanxia, XIE Xuewen, CAO Jinqiang, CHAI Ali, LI Baoju. Molecular Detection and Analysis of Main Viruses of Tomato in China [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(1): 185-193.
[7]	LI Xin, CUI Xiuming, LIU Diqiu, SUN Tian, GUO Ailing, CHEN Jun. Real-time PCR Detection Method of Panax notoginseng Root Rot Pathogen Fusarium oxysporum [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(S1): 324-330.
[8]	ZHANG Jingjing, ZHANG Haiying, PAN Xiuqing, XU Yong, REN Yi, GAO Xiurui, LI Bing, SHI Yufan, DANG Jige, YANG Mingzhu, WU Yanrong. KASP Assays of Disease Resistance Genes from 130 Watermelon Varieties and Germplasms in Hebei Province [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(2): 110-116.
[9]	YANG Rui, HAN Xuechun, LI Qing, CHEN Zhaorong. Screening of Pear Viral Disease Detection Methods and Detection of Pear Virus Disease in Tianjin Region [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(5): 125-129.
[10]	ZHANG Junhuan, SUN Haoyuan, Boris Krška, YANG Li, JIANG Fengchao, ZHANG Meiling, WANG Yuzhu. *RT-PCR Detection of Plum pox virus* and the Screening of DNA Markers Linked to PPV-resistance in Apricot** [J]. Acta Agriculturae Boreali-Sinica, 2018, 33(4): 31-38.
[11]	REN Shilong, YANG Zhenli, BAI Hui, WANG Yongfang, QUAN Jianzhang, DONG Zhiping, LI Zhiyong, XING Jihong. *Cloning of Mating Type Genes from Foxtail Millet Blast and Molecular Detection of Magnaporthe oryzae* Mating Type Genes in Different Region** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(3): 56-62.
[12]	SONG Zhicheng, FEI Xinmin, YANG Yu, QIAO Lixian, WANG Jingshan, LI Guangcun, GUO Baotai. *Preparation of Polyclonal Antibody Against Recombinant CP of Potato virus A* and Its Application to DAS-ELISA Detection** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(1): 41-46.
[13]	QIAN Zhukai, ZHANG Chuanqing, ZHOU Gen, DAI Dejiang, SHI Haojie. Establishment of Loop-mediated Isothermal Amplification Assay to Rapidly Detect Carbendazim High Resistant Strain of Botrytis cinerea [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(S1): 438-442.
[14]	HONG Chunhui, YANG Bing, TAN Xiangjie, ZHOU Hongzhuan, XU Fuzhou, SU Xia, XUE Jing, ZHANG Xiaodong, WANG Jinluo. Development of a Triple PCR Method for Identifying Three Genotypes of Porcine bocavirus [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(2): 12-16.
[15]	XU Yuan, LI Lingxian, WEI Chunru, WEI Xinyan, YU Xiumei, LIU Daqun. *Cloning and Construction of Yeast Two-hybrid Bait Vector of Wheat Ta-SKP2A* Gene** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(2): 17-22.

Please choose a citation manager

Content to export