Genomic DNA Extraction and RAPD Analysis of Cucumber

doi:10.3321/j.issn:1000-7091.2002.04.003

Abstract

Abstract: The preparat ion of DNA samples with good quality is the basis for researches of plant molecular biology.The DNA samples of cucumber male sterile line YS and maintainer line MC were prepared by SDS, Urea, Improved SDS, Benzyl chloride and CTAB method, respectively. The results showed that DNA samples obtained by improved SDS method possessed the standard ultraviolet absorbance spectrum of the pure natural DNA and the A₂₆₀/ A₂₈₀ was between 1.7- 1.9, the yield of DNA obtained by improved SDS method was 450Lg/g young leaves, and the DNA samples prepared by this procedure were suitable for RAPD analysis.

Key words: Cucumber, DNA ext ract ion, RAPD analysis

CLC Number:

S62.2

LIU Dian-lin, YANG Rui-huan, HA Yu-jie. Genomic DNA Extraction and RAPD Analysis of Cucumber[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2002, 17(4): 9-12. doi: 10.3321/j.issn:1000-7091.2002.04.003.

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