ACTA AGRICULTURAE BOREALI-SINICA ›› 1999, Vol. 14 ›› Issue (4): 60-62. doi: 10.3321/j.issn:1000-7091.1999.04.012

Special Issue: Multigrain crops

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Selection of Millet Resistance Concentration of Hygromycin

Wang Jiezhi, Hao Xiaofen, Zheng Xiangyang, Wang Luying, Sun Meirong   

  1. Millet Research Institute Shanxi Academy of Agricultural Sciences, Changzhi 046011
  • Received:1998-07-28 Published:1999-12-28

Abstract: The result of experiment showed that callus differentiation regrowthes and roots become sensitivity when the resistance concentration of hygromycin in culture medium reached 5 mg/L.The critical concentration is 10 mg/L.Husked millet become sensitivity when the concentration reached 10 mg/L.The critical concentration is 15 mg/L.The resistance concentration of hygromycin selected is the basis for testing transgenes millet plant.

Key words: Millet, Tissue culture, Resistance concentration, Hygromycin

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Cite this article

Wang Jiezhi, Hao Xiaofen, Zheng Xiangyang, Wang Luying, Sun Meirong. Selection of Millet Resistance Concentration of Hygromycin[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1999, 14(4): 60-62. doi: 10.3321/j.issn:1000-7091.1999.04.012.

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