RAPD Analysis on Different Nullisomic Genomes of Wheat“Chinese Spring”

doi:10.3321/j.issn:1000-7091.1996.03.006

Abstract

Abstract: RAPD analysis on seven nullisomic genomes of wheat “Chinese Spring”was conducted using four primers with 10mer oligonucleotides(OPT 13,OPT 14,OPT 6 and OPT 7).The RAPD products revealed that the amplfied DNA bands fell into two categories:those could be amplified in all genomes,represented the DNA stability in evolution;and those differed in the studied genomes,were the important bands for genome identification.Results also suggested that RAPD“fingerprints”assay of nuclear DNA could be used as a tool in the study of systematic evolution of wheat.

Key words: Nullisomic wheat Chinese Spring, Nuclear genome, RAPD

CLC Number:

S512.1

He Congfen, Huang Zhanjing, Shen Yinzhu, Liu Zhiyi, Bai Feng, Ma Wenshi, Zhang Zhaoduo. RAPD Analysis on Different Nullisomic Genomes of Wheat“Chinese Spring”[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1996, 11(3): 31-34. doi: 10.3321/j.issn:1000-7091.1996.03.006.

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