CMVcp Gene Transformation and Expression in Pepper

doi:10.3321/j.issn:1000-7091.1994.03.015

Abstract

Abstract: Cotyledon sxplants from three pepper cultivars were co-cultured with an agrobacterium strain carrying a binary Ti vector in which a foreign CMV_cp gene was inserted after the 35 spromotor After kanamycin-resistent selection and regeneration culture five transgeneplants and their offsprings were obtained.The transformation rate is 10%.Results of Southern and Northern Blots as well as ELISA tests indicated that the CMV_cp gene has incorperated, transcripted and translated in transgeneplants and their offsprings.Virus inoculation tests prove the resistance of the transgeneplants and their offsprings to CMV virus.

Key words: Pepper, CMV_cp, Gene transformation

CLC Number:

S188

Zhang Zongjiang, Zhou Zhongxin, Liu Yanjun, Jing Qianyun, You Ming, Liu Guomin, Mi Jingjiu. CMV_cp Gene Transformation and Expression in Pepper[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1994, 9(3): 67-71. doi: 10.3321/j.issn:1000-7091.1994.03.015.

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