Preliminary Study on Regeneration Induction and Gene Transformation of CMVcp in Pepper

doi:10.3321/j.issn:1000-7091.1991.04.012

Abstract

Abstract: A program suitable for pepper plantlets regeneration has been developed. Thallus brushes, buds and roots were induced from explants of pepper cotyledon on MS + 5 mg 6BA + 0.1mgIBA, MS + 3 mg6 BA + 0.2mgIBA and MS + 0.2mgIAA, respectively. Thereby, got whole plantlet. Explants from Pepper cotyledon were inoculated with A, tumefaciens cells containing a cointegrate Ti-plastid which carries NPTⅡ , Nopaline syntheses and CMVcp genes.Transformed cells were selected for kanamycin resistance and transgene plantlets has been gained after a regeneration culture.

Key words: Pepper, Regenerative plantlet, CMVcp, Gene transformation, Tissue culture

ZhouZhongxin, SuMilan, ChenDefen, SongLanying, YangJinghui. Preliminary Study on Regeneration Induction and Gene Transformation of CMVcp in Pepper[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1991, 6(4): 69-72. doi: 10.3321/j.issn:1000-7091.1991.04.012.

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