华北农学报 ›› 2006, Vol. 21 ›› Issue (6): 26-29. doi: 10.3321/j.issn:1000-7091.2006.06.007

所属专题: 水稻

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水稻胚囊发育过程中特异类型的共聚焦显微镜观察

代西梅, 黄群策, 秦广雍, 李国平   

  1. 郑州大学物理工程学院离子束生物工程省重点实验室, 河南郑州 450052
  • 收稿日期:2006-01-12 出版日期:2006-12-28
  • 通讯作者: 黄群策
  • 作者简介:代西梅(1974- ),女,河南郑州人,讲师,博士,主要从事植物遗传育种研究工作。
  • 基金资助:
    国家“863”项目(SZ-01-01-03);国家“十五”科技攻关项目(2001BA302B)

Observation on Particular Embryo Sac of Rice Using Confocal Microscopy

DAI Xi-mei, HUANG Qun-ce, QIN Guang-yong, LI Guo-ping   

  1. Provincial Key Laboratory of Ion Beam Bioengineering, Physical Engineering College of Zhengzhou University, Zhengzhou 450052, China
  • Received:2006-01-12 Published:2006-12-28

摘要: 利用激光扫描共聚焦显微镜术对水稻品种IR36二倍体及其同源四倍体的胚囊形成和发育过程进行了系统的研究。在IR36二倍体胚囊的发育过程中,观察到了几种前人未曾报道的异常胚囊类型,即三核胚囊、六核胚囊、九核胚囊及一些退化胚囊,其形成机制有待进一步研究。观察结果可为正确认识水稻胚囊发育形成过程提供参考资料。

关键词: 水稻, 胚囊, 激光扫描共聚焦显微镜, 发育

Abstract: The formation and development of embryo sac in the diploid and its autotetraploid of rice cultivar IR36 was examined using Laser Scanning Confocal Microscopy.Some particular types of embryo sacs,such as those with three-nucleare,six-nucleare and nine-nucleare,and degenerated ones,were found in diploid rice.These types of embryo sacs were not reported before.The mechanism for the formation of these specific embryo sacs is not clear and needs to be studied further.Our results provide some scientific references for better understanding the development of rice embryo sac.

Key words: Rice, Embryo sac, Laser Scanning Confocal Microscope(LSCM), Development

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引用本文

代西梅, 黄群策, 秦广雍, 李国平. 水稻胚囊发育过程中特异类型的共聚焦显微镜观察[J]. 华北农学报, 2006, 21(6): 26-29. doi: 10.3321/j.issn:1000-7091.2006.06.007.

DAI Xi-mei, HUANG Qun-ce, QIN Guang-yong, LI Guo-ping. Observation on Particular Embryo Sac of Rice Using Confocal Microscopy[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(6): 26-29. doi: 10.3321/j.issn:1000-7091.2006.06.007.

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