Acta Agriculturae Boreali-Sinica ›› 2022, Vol. 37 ›› Issue (2): 35-41. doi: 10.7668/hbnxb.20192531

Special Issue: Hot Article

• Crop Genetics & Breeding·Germplasm Resources·Biotechnology • Previous Articles     Next Articles

Application of VNTR-specific Primers in Fertility Identification of Sugar Beet

ZHANG Hui, WANG Liang, FU Zengjuan, LI Xiaodong, ZHAO Shangmin, E Yuanyuan, ZHENG Wenzhe, ZHANG Ziqiang, ZHANG Bizhou, ZHANG Huizhong   

  1. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China
  • Received:2021-11-22 Published:2022-04-28

VNTR特异性引物在甜菜育性鉴定中的应用

张辉, 王良, 付增娟, 李晓东, 赵尚敏, 鄂圆圆, 郑文哲, 张自强, 张必周, 张惠忠   

  1. 内蒙古自治区农牧业科学院,内蒙古 呼和浩特 010031
  • 作者简介:
    作者简介:张 辉(1985—),男,河北沧县人,副研究员,博士,主要从事甜菜遗传育种与生物技术研究。
  • 基金资助:
    现代农业产业技术体系建设专项(CARS-170104)

Abstract:

In order to find out the cytoplasm types of some sterile line and maintainer line materials in the process of sugar beet breeding, it provides a guiding direction for the next breeding work. Molecular marker primer TR1 was used to identify the molecular level of 30 test materials cytoplasm types, and to compare the fertility traits in the field. The results showed that the PCR electrophoresis bands of maintainer line 960767 and sterile line N9848 were 750,500 bp.We named these cytoplasm types as N1 and S,respectively.And 750 bp bands were detected by T152,T328,T334,T306,T360,as N1 cytoplasm.The 500 bp bands detected were T766,T154,N122,I-1,B-3,Z-1,41419,10320,B-3-23,S865,S301,S151,S327,S333,S115,S305,S153,S359,S137,S163,and S313,inferred to be S type cytoplasm.The materials for the bands detected between 500 bp and 750 bp were T302,and T116.Because T302 also had the ability to maintain the sterility of S301 in field investigations,T302 and T116 were classified into another type,N2 cytoplasm type.Some plants with 500, 750 bp of materials were T302, I-1 and B-3, which included both normal and Owen cytoplasm were characteristic materials. The results of field pollen fertility survey T766, T154, N122, I-1, B-3, Z-1, 414191, 10320 and B-3-23 were all fertile, but their cytoplasm type was identified to belong to S type cytoplasm. According to molecular identification and field fertility trait survey, the results of most materials were consistent with the results of fertility trait survey. Some materials had inconsistent molecular identification and fertility trait results, requiring further adjustment of the selection direction.

Key words: Sugar beet, Molecular markers, Fertility identification, TR1 primer

摘要:

为了摸清甜菜选育进程中部分不育系和保持系材料的细胞质类型,为下一步的选育工作提供指导方向。利用分子标记引物TR1对国内总共30份甜菜试验材料开展细胞质类型的分子水平鉴定,并结合田间花期育性性状调查进行比对。结果表明,成型稳定的保持系960767和不育系N9848经过TR1引物鉴定后的PCR电泳条带分别为750,500 bp,其胞质类型分别为N1和S型,其他试验材料检测到750 bp的材料为T152、T328、T334、T306、T360,推断其为N1型细胞质;检测到500 bp条带的材料为T766、T154、N122、I-1、B-3、Z-1、41419、10320、B-3-23、S865、S301、S151、S327、S333、S115、S305、S153、S359、S137、S163和S313,推断其为S型细胞质;检测到500~750 bp条带的材料为T302、T116。由于T302在田间调查中其对S301的不育性也具有保持能力,因此将T302和T116分为N型细胞质的另一种类型N2。同时检测到500 bp和750 bp的材料为T302、I-1和B-3的部分植株,为同时包括正常和欧文细胞质的材料,属于特质性材料。田间花粉育性调查结果T766、T154、N122、I-1、B-3、Z-1、41419、10320、B-3-23均为可育型,但鉴定其胞质类型属于S型细胞质。经分子鉴定和田间育性性状调查对比,大部分材料分子鉴定结果与育性性状调查结果一致。部分材料存在分子鉴定与育性性状结果不一致,有待于进一步调整选育方向。

关键词: 甜菜, 分子标记, 育性鉴定, TR1引物