Inoculation with Arbuscular Mycorrhizal Fungi Enhances Drought Tolerance in Medicinal Tree Peony

doi:10.7668/hbnxb.20196636

Abstract

Abstract:

This study investigated the effects of arbuscular mycorrhizal fungi(AMF)inoculation on the physiological characteristics and the accumulation of active components in the medicinal tree peony Paeonia ostii Fengdan under drought stress,with the aim of providing a theoretical basis for utilizing AMF to enhance the drought resistance and medicinal quality of this species.Using P.ostii seedlings as experimental material,four treatments were established:well-watered control(CK),drought stress(DR),AMF inoculation under well-watered conditions(AMCK),and AMF inoculation under drought stress(AMDR).Indicators related to plant redox homeostasis(including superoxide dismutase(SOD)activity,proline and soluble sugar content),photosynthetic characteristics(including photosynthetic pigment content,gas exchange parameters,and chlorophyll fluorescence parameters),and the content of active components(such as total flavonoids,paeonol,and paeoniflorin)in the root bark were measured.The results showed that drought stress induced significant accumulation of malondialdehyde(MDA)and reactive oxygen species(ROS),with increases of 33.53% and 50.04%, respectively,while photosynthetic performance was markedly inhibited.This was specifically evidenced by significant decreases in net photosynthetic rate(Pn),stomatal conductance(Gs),transpiration rate(Tr),maximum photochemical efficiency(Fv/Fm),and photosynthetic performance index(PIabs).AMF inoculation effectively alleviated these adverse effects.It was found that inoculation not only significantly increased SOD activity by 6.36% and elevated the contents of osmoregulatory substances (soluble proteins, soluble sugars, and proline) by 23.72%, 53.31%, and 14.70%, respectively,but also reduced ROS levels,thereby enhancing cell membrane stability.A significant recovery was also observed in photosynthetic pigment content and photosynthetic and chlorophyll fluorescence parameters(Pn,Gs,Tr,Fv/Fm,and PIabs).Furthermore,under drought stress conditions,AMF inoculation further promoted the accumulation of secondary metabolites in the root bark,including total flavonoids,paeonol,and paeoniflorin,with increases of 18.67%, 11.39%, and 5.17%, respectively, compared with the DR treatment.In conclusion,it is demonstrated that AMF can effectively mitigate the physiological damage caused by drought stress in P.ostii by enhancing its antioxidant capacity,promoting the accumulation of osmoregulatory substances,and improving photosynthetic performance.Simultaneously,AMF boosts the content of secondary metabolites in the root bark.

Key words: Medicinal peony, Arbuscular mycorrhizal fungi, Drought stress, Photosynthetic characteristics, Active components, Antioxidant capacity

CLC Number:

S318其他栽培技术与方法

CHEN Ke, ZHANG Biao, ZHANG Youfu, WANG Zi, GONG Minggui, CHANG Qingshan, SUN Yiming, ZHANG Lixia, HOU Xiaogai. Inoculation with Arbuscular Mycorrhizal Fungi Enhances Drought Tolerance in Medicinal Tree Peony[J]. Acta Agriculturae Boreali-Sinica, 2026, 41(4): 140-151. doi: 10.7668/hbnxb.20196636.

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Figures/Tables 9

Tab.1 AMF colonization rate under different treatments

处理 Treatment	AMF侵染率/% AMF colonization rate
CK	18.52±4.49c
DR	10.74±1.70d
AMCK	81.11±1.11a
AMDR	60.37±2.31b

Fig.1 Effects of AMF on SOD activity and the contents of soluble protein,soluble sugar and proline in P. ostii leaves under drought stress Different lowercase letters indicate significant differences between different treatments(P<0.05).The same as Fig.2—7.

Fig.2 Effects of AMF on relative electrical conductivity and the contents of MDA,H₂O₂ and $\mathrm{O}^{\bar{.}}_{2}$ in P. ostii leaves under drought stress

Fig.3 Effects of AMF on the content of photosynthetic pigments in P. ostii leaves under drought stress

Tab.2 Effects of AMF on gas exchange parameters in P.ostii leaves under drought stress

处理 Treatment	Pn/ (μmol/(m²·s))	Gs/ (mol/(m²·s))	Ci/ (μmol/mol)	Tr/ (mmol/(m²·s))
CK	6.63±0.12b	0.048±0.001c	155.93±2.37c	1.47±0.04b
DR	4.63±0.08d	0.041±0.001d	192.50±3.09a	1.38±0.02c
AMCK	7.45±0.04a	0.052±0.001a	142.88±2.78d	1.56±0.02a
AMDR	6.21±0.04c	0.050±0.000b	176.48±0.85b	1.52±0.01ab
干旱 Drought stress	^**	^**	^**	^**
AMF	^**	^**	^**	^**
干旱Drought stress×AMF	^**	^**	NS	NS

Fig.4 Effects of AMF on W_K,V_J,M₀,and φE₀ in P.ostii leaves under drought stress

Fig.5 Effects of AMF on Fv/Fm and PIabs in P.ostii leaves under drought stress

Fig.6 Effects of AMF on the content of total flavonoids in P.ostii root bark under drought stress

Fig.7 Effects of AMF on the contents of active components in P.ostii root bark under drought stress

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