The Physiological Characteristics and Expression Differences of Related Genes of Yellow-seeded Rapeseed with Different Seed Colors

doi:10.7668/hbnxb.20195526

Abstract

Abstract:

Yellow-seeded rapeseed is popular with consumers because of the appearance and good quality of rapeseed oil.However,the unstable separation of progeny traits seriously affects its large-scale application.In order to analyze the internal reasons for the unstable separation of yellow-seeded rapeseed traits,the differences in the internal physiological mechanisms between yellow and black seeds in yellow-seeded rapeseed were explored.Yellow-seeded rapeseed(CK)was used as the material to study the differences in agronomic traits,physiological and biochemical indexes,and seed coat color-related genes between yellow(Y)and black(B)seed plants in the separation offspring.The results showed that the rhizome diameter and plant height of Y were larger than those of CK and B.The plant height of B was significantly different from that of CK and Y,respectively,and the rhizome diameter of B was significantly different from that of Y.The disease index of Y was 1.97,and the disease indexes of CK and B were 2.55 and 3.33,respectively,indicating that Y was superior to CK and B in disease resistance.The content of MDA in Y leaves was the lowest at 9—10 leaf stage,and the POD activity in Y and CK flowers continued to increase at flowering stage,indicating that yellow-seeded rape had strong stress resistance.The expression levels of TT18 and TT8 genes in B and Y at 7—8 leaf stage and 9—10 leaf stage were higher than those in CK,and the expression levels of TT18 gene in B and Y at final flowering stage were significantly lower than those in CK.At 28 days after pollination,the expression of MYB47 gene in Y seed was the highest,which was 5.56 times that of CK and 5.79 times that of B,respectively.The expression of TT8 gene was the highest in Y at 21 days after pollination,which was 3.30,2.29 times that of CK and B,respectively.It showed that yellow-seeded rapeseed had obvious advantages in oil content and stress resistance.Therefore,vigorous development of yellow-seeded rapeseed can provide new ideas for improving the supply of rapeseed oil and solving the safety of edible oil in China.

Key words: Brassica napus L., Yellow seed, Peroxidase, Transparent Testa 8

CLC Number:

S565.4

ZHANG Qi, TIAN Yantao, XIAO Gang, ZHANG Zhenqian. The Physiological Characteristics and Expression Differences of Related Genes of Yellow-seeded Rapeseed with Different Seed Colors[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(5): 96-104. doi: 10.7668/hbnxb.20195526.

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Figures/Tables 10

References 41

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基因名称 Gene name	正向引物序列(5'—3') Forward primer(5'—3')	反向引物序列(3'—5') Reverse primer(3'—5')
Actin	CGTTGGTGGAGTTGCACTTG	AGCACGTTACGGGATTGGTT
MYB47	GTCGAGGAAAGGAATTGACCCG	CCTTCTTCACCACCACCAATCGTGT
TT18	CTGATTCGATTGTGATGCACAT	TCCTTACCTTCTCCTTGTTCAC
PDAT1	TCCATCCTCGTTCTGTTATCC	CACCGACGACTGATGAAACGA
TT8	TTAAGGCGGTGGTGCAATCT	GCGCCGTTGTAGTATCCACT

基因名称 Gene name	正向引物序列(5'—3') Forward primer(5'—3')	反向引物序列(3'—5') Reverse primer(3'—5')
Actin	CGTTGGTGGAGTTGCACTTG	AGCACGTTACGGGATTGGTT
MYB47	GTCGAGGAAAGGAATTGACCCG	CCTTCTTCACCACCACCAATCGTGT
TT18	CTGATTCGATTGTGATGCACAT	TCCTTACCTTCTCCTTGTTCAC
PDAT1	TCCATCCTCGTTCTGTTATCC	CACCGACGACTGATGAAACGA
TT8	TTAAGGCGGTGGTGCAATCT	GCGCCGTTGTAGTATCCACT

材料 Materials	主茎绿叶数/个 Green leaf number on main stalks	主茎总叶数/个 Total leaf number on main stalks	最大叶The largest leaf		根茎粗/cm Width of rootstock	株高/cm Plant height
材料 Materials	主茎绿叶数/个 Green leaf number on main stalks	主茎总叶数/个 Total leaf number on main stalks	长/cm Length	宽/cm Width	根茎粗/cm Width of rootstock	株高/cm Plant height
CK	8.00±1.22a	9.00±1.22a	20.18±3.33a	13.16±1.36a	1.12±0.13ab	15.38±4.02a
B	10.20±1.79a	11.60±1.82a	18.75±1.69a	11.84±1.30a	0.73±0.25b	8.93±3.46b
Y	10.00±0.71a	11.20±0.84a	18.90±0.95a	12.52±1.49a	1.36±0.40a	17.00±1.22a

材料 Materials	主茎绿叶数/个 Green leaf number on main stalks	主茎总叶数/个 Total leaf number on main stalks	最大叶The largest leaf		根茎粗/cm Width of rootstock	株高/cm Plant height
材料 Materials	主茎绿叶数/个 Green leaf number on main stalks	主茎总叶数/个 Total leaf number on main stalks	长/cm Length	宽/cm Width	根茎粗/cm Width of rootstock	株高/cm Plant height
CK	8.00±1.22a	9.00±1.22a	20.18±3.33a	13.16±1.36a	1.12±0.13ab	15.38±4.02a
B	10.20±1.79a	11.60±1.82a	18.75±1.69a	11.84±1.30a	0.73±0.25b	8.93±3.46b
Y	10.00±0.71a	11.20±0.84a	18.90±0.95a	12.52±1.49a	1.36±0.40a	17.00±1.22a

农艺性状 Agronomic traits	主茎绿叶数 Green leaves of main stem	主茎总叶数 Total leaves of main stem	最大叶长 Maximum leaf length	最大叶宽 Maximum leaf width	根茎粗 Width of rootstock	株高 Plant height
主茎绿叶数Green leaves of main stem	1.000
主茎总叶数Total leaves of main stem	0.998^*	1.000
最大叶长Maximum leaf length	-1.000^**	-0.999^*	1.000
最大叶宽Maximum leaf width	-0.897	-0.922	0.902	1.000
根茎粗Width of rootstock	-0.217	-0.276	0.230	0.627	1.000
株高Plant height	-0.403	-0.458	0.415	0.767	0.981	1.000

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