Responses of Mesophyll Conductance and Photosynthetic Biochemical Parameters of Apples to Different Light Environments in Canopy

doi:10.7668/hbnxb.20195841

Abstract

Abstract:

In order to explore the effects of different canopy light environments on apple mesophyll conductance and its key parameters and photosynthetic biochemical parameters,the open-center Fuji apple trees were used as experimental materials.The canopy light environment,photosynthetic biochemical parameters and leaf tissue structure of apple trees were measured after thinning,and the apple trees without thinning were used as controls(CK).Results showed that,the peak and mean value of daily maximum and averaged photosynthetically active radiation(PAR)increased by 23%,166%,and 43%,123%,respectively.Compared to the CK,product of light absorption coefficient and light energy partitioning ratio(α·β)and dark respiration rate under light(R_d)of treatment increased by 84.62%,56.00% respectively,while apparent CO₂ compensation point(C_i^*)value decreased by 6.67%.The average value of mesophyll conductance(g_m)calculated by quantitative α·β value was 9.89% higher than that of the CK,and the maximum and average values of mesophyll conductance(g_m')calculated by empirical α·β value(0.425)were 20.65%,39.38% higher than those of the CK,respectively.The g_m ' of the treatment and the CK was higher than that of the g_m,and the g_m' of the treatment and the CK was 54.33%,21.67% higher than the average value of the g_m,respectively.V_cmax derived from P_n-C_i(neglecting mesophyll conductance),P_n-C_c'(using empirical α·β)and P_n-C_c(using quantified α·β)response curves demonstrated significant differences(V_cmax-_C_i and V_cmax-_C_c' underestimated by 40.59%,19.85% in comparison with V_cmax-_C_c in treatment,respectively),and treatment was significantly higher than CK(V_cmax-_C_i,V_cmax-_C_c' and V_cmax-_C_c increased by 11.21%,5.85%,10.69%,respectively);J_max and V_tpu were similar to V_cmax.The anatomical structures of leaves showed that the mean values of palisade tissue(PT)thickness and spongy tissue(ST)thickness of thinning treatment significantly increased respectively by 43.95%,43.31%,in comparison with those of the CK.The area and perimeter of PT and ST in thinning treatment were respectively 43.60% and 310.86% with 34.73% and 115.82% higher than those in CK.In summary,after thinning treatment,the light condition of apple canopy was improved,the leaf structure was changed,and the photosynthetic efficiency of the plant was improved.

Key words: Apple, Tree thinning, Canopy, Light environment, Mesophyll conductance, Photosynthetic biochemical parameters

CLC Number:

S661.1

LI Zhiqiang, LI Mi, LIN Lu, WANG Hongning, WANG Pengpeng, ZHAO Guoping. Responses of Mesophyll Conductance and Photosynthetic Biochemical Parameters of Apples to Different Light Environments in Canopy[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(5): 105-116. doi: 10.7668/hbnxb.20195841.

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Figures/Tables 9

Fig.1 Difference analysis of canopy interception of photosynthetically active radiation(PAR) under different treatments

Fig.2 Difference analysis of mesophyll conductance parameters under different treatments Different lowercases indicated significant differences between different treatments.The same as Fig.6,8.

Fig.3 Differences in g_m-PAR and g_m'-PAR response curves of different treatments Different lowercases indicated significant differences between g_m and g_m' under the same PAR(P<0.05).

Fig.4 Differences in P_n-C_i, P_n-C_c and P_n-C_c' response curves of different treatments Different lowercases indicated significant differences of P_n-C_i,P_n-C_c,and P_n-C_c' response curves between tree-thinning treatment and CK(P<0.05).

Tab.1 The different of photosynthetic biochemical parameters fitted by P_n-C_i,P_n-C_c' and P_n-C_c response curves among different treatments μmol/(m²·s)

处理 Treatment	V_cmax-_C_i	V_cmax-_C_c'	V_cmax-_C_c	J_max-_C_i	J_max-_C_c'	J_max-_C_c	V_tpu-_C_i	V_tpu-_C_c'	V_tpu-_C_c
间伐Tree-thinning	135.01±2.95c^*	182.15±2.64b^*	227.26±4.61a^*	141.03±4.26c	237.67±3.25b^*	251.84±3.95a^*	13.48±0.19a^*	10.21±0.22b^*	8.49±0.23c^*
对照CK	121.40±1.70c	172.08±3.82b	205.32±5.97a	138.24±3.96c	214.03±5.35b	225.26±5.78a	9.98±0.10a	8.88±0.13b	7.05±0.14c

Fig.5 Semi-thin sections of leaf tissue of tree-thinning treatment and CK

Fig.6 Differences in different tissues of mesophyll cells between tree-thinning treatment and CK

Fig.7 Ultrathin slices photographs for leaf anatomical structures of tree-thinning treatment and CK

Fig.8 Differences in the areas and perimeters of palisade tissue and spongy tissue in mesophyll cells of tree-thinning treatment and CK

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