Effects of Amino Acid Type Water-soluble Fertilizer on Chlorophyll Content and Expression of Genes Related to Metabolism in Celery at Seedling Stage

doi:10.7668/hbnxb.20195431

Abstract

Abstract:

Explore the effects of amino acid type water-soluble fertilizer on chlorophyll content and proportion of celery,and the regulation of gene expression related to chlorophyll metabolism,which provides a theoretical basis for the rational application of amino acid type water-soluble fertilizer foliar fertilizer in the production of high-quality pigment-rich celery.The celery variety Ningqin 1 was sprayed with different concentrations of amino acid type water-soluble fertilizer,the contents of chlorophyll a,chlorophyll b and total chlorophyll in leaf blades and petioles were determined and analyzed,and the relative expression levels of genes related to chlorophyll metabolism were determined by fluorescence quantitative PCR(RT-qPCR).The effects of amino acid type water-soluble fertilizer on chlorophyll accumulation and relative expression levels of related genes were related to the treatment concentration and leaf location.Treatment with 500 μL/L amino acid type water-soluble fertilizer increased the contents of chlorophyll a,chlorophyll b and total chlorophyll in leaf blades and petioles,and decreased the ratio of chlorophyll a/b in petioles.The expression of chlorophyll synthesis-related genes(AgHEMA,AgHEMB,AgCHLM,AgPOR and AgCAO)was induced,and the expression levels of degradation related genes(AgPAO and AgPPH)were significantly down-regulated.Treatment with 1 000 μL/L amino acid type water-soluble fertilizer inhibited chlorophyll accumulation to a certain extent,increased chlorophyll a/b ratio in leaf blades,and significantly down-regulated the expression levels of genes related to chlorophyll synthesis and chlorophyll degradation AgPPH.The suitable concentration of amino acid type water-soluble fertilizer foliar fertilizer can improve the chlorophyll content of celery at seeding stage by regulating the expression level of genes related to chlorophyll metabolism.

Key words: Celery, Amino acid type water-soluble fertilizer, Foliar spray, Chlorophyll metabolism, Seedling stage

CLC Number:

S636.3

YUAN Xueyun, CHEN Chen, YAN Yue, WEI Yongju, WANG Lixiang, SUN Xu, SUN Yuehua, LI Yapeng, LIU Peizhuo, XIONG Aisheng. Effects of Amino Acid Type Water-soluble Fertilizer on Chlorophyll Content and Expression of Genes Related to Metabolism in Celery at Seedling Stage[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(3): 97-103. doi: 10.7668/hbnxb.20195431.

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Figures/Tables 5

Fig.1 Phenotypes of celery under different treatments Ⅰ.HCK(60 d);Ⅱ.H0(65 d);Ⅲ.H1(65 d);Ⅳ.H2(65 d)。

Tab.1 Agronomic traits of celery under different treatments

处理 Test	叶柄数/个 Number of petiole	株高/cm Plant height	单株质量/g Single plant weight	根长/cm Root length
HCK	3.33±0.58c	8.93±0.83b	1.32±0.33c	5.07±0.52b
H0	3.67±0.58bc	10.63±2.29ab	1.78±0.58bc	5.95±0.87b
H1	4.67±0.58ab	10.97±1.09ab	2.22±0.28ab	7.89±0.85a
H2	5.33±0.58a	12.23±1.33a	2.98±0.36a	7.99±0.62a

Fig.2 Effects of amino acid type water-soluble fertilizer treatment on chlorophyll content and proportion in celery leaf blades and petioles The data in each graph were grouped according to celery parts(leaf blades,petioles) and then analyzed for the difference significance;different lowercase letters indicated significant differences(P<0.05).

Fig.3 Effect of amino acid type water-soluble fertilizer treatment on relative expression levels of genes related to chlorophyll synthesis in celery leaf blades and petioles The data in each graph were grouped by gene and analyzed for difference significance; different lowercase letters indicated significant difference(P<0.05).The same as Fig.4.

Fig.4 Effect of amino acid type water-soluble fertilizer treatment on relative expression levels of genes related to chlorophyll degradation in celery leaf blades and petioles

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