Analysis of FITM2 Gene Polymorphism and Its Association with Growth Traits in Hu Sheep

doi:10.7668/hbnxb.20195349

Abstract

Abstract:

Fat storage inducing transmembrane protein 2 (FITM2) plays an important role in regulating lipid storage and skeletal muscle energy metabolism.To scan the polymorphisms of the FITM2 gene in sheep and investigate its relationship with growth traits in Hu sheep,the experiment selected 1 128 healthy and disease-free,genetically well-documented Hu sheep as the experimental group,and used the FITM2 gene in sheep as the research object.First,the expression differences of the FITM2 gene in different tissues were analyzed using qPCR technology.Then,the genetic polymorphisms of the FITM2 gene in sheep were detected using PCR amplification,Sanger sequencing,and AQP (allele-specific quantitative PCR-based genotyping assay).Finally,the association between the genetic polymorphisms and growth traits was analyzed.The research results showed that the FITM2 gene was expressed in different tissues of Hu sheep,with the highest expression level in the tail fat and significantly higher than that in other tissues.The gene polymorphism detection results showed that there was a C>T mutation at position 72704027 in the first intron of the FITM2 gene.The results of correlation analysis showed that the 100,120 d body weight,140,160 d body height,80,120,140,160 d body length,and 100 d chest circumference of CC genotype individuals were significantly higher than those of TT genotype individuals.In summary,the FITM2 gene g.72704027 C>T mutation site in sheep can be used as a candidate molecular marker related to growth traits in Hu sheep,providing a theoretical basis for the genetic breeding work of Hu sheep.

Key words: Hu sheep, SNP, FITM2, Association analysis, Tissue expression profile

CLC Number:

813.3

KONG Dewen, WANG Weimin, TIAN Huibin, ZHANG Deyin, ZHAO Liming, YANG Xiaobin, MA Zongwu, LI Chenghai, ZHANG Jian, PU Mengru, CAO Peiliang, LI Linting, LI Hongjian, JIA Guoxing, GAO Fei, WU Wanen, WANG Lizhong, FENG Lianjun, XIAO Ziyue, ZHANG Qi, YAN Chengqi, GAO Lei, ZHANG Xiaoxue. Analysis of FITM2 Gene Polymorphism and Its Association with Growth Traits in Hu Sheep[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(3): 173-181. doi: 10.7668/hbnxb.20195349.

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References 40

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引物名称 Primer name	引物序列(5'—3') Primer sequence(5'—3')	产物大小/bp Product size	Tm/℃	用途 Usage
FITM2-F	CCAGCCCAGCAGACCGAAC	694	58.30	PCR
FITM2-R	ACGCTTTCACTTTCTTGAGGT
R-FITM2-F	TGGCACGGCTTCGACATCTCA	105	60.95	qPCR
R-FITM2-R	GTTCCGGTCCGTCTTCACCT
UXT-F	GCAAGTGGATTTGGGCTGTAAC	180	58.51	内参基因
UXT-R	ATGGAGTCCTTGGTGAGGCTGT
FITM2-Fam	GAAGGTGACCAAGTTCATGCTGTGAAGAGC			AQP
	AGGGATGTGGC
FITM2-Hex	GAAGGTCGGAGTCAACGGATTGGTGAAGAG
	CAGGGATGTGGT
FITM2-Rev	GGCATGACACGGGACTCTCC

引物名称 Primer name	引物序列(5'—3') Primer sequence(5'—3')	产物大小/bp Product size	Tm/℃	用途 Usage
FITM2-F	CCAGCCCAGCAGACCGAAC	694	58.30	PCR
FITM2-R	ACGCTTTCACTTTCTTGAGGT
R-FITM2-F	TGGCACGGCTTCGACATCTCA	105	60.95	qPCR
R-FITM2-R	GTTCCGGTCCGTCTTCACCT
UXT-F	GCAAGTGGATTTGGGCTGTAAC	180	58.51	内参基因
UXT-R	ATGGAGTCCTTGGTGAGGCTGT
FITM2-Fam	GAAGGTGACCAAGTTCATGCTGTGAAGAGC			AQP
	AGGGATGTGGC
FITM2-Hex	GAAGGTCGGAGTCAACGGATTGGTGAAGAG
	CAGGGATGTGGT
FITM2-Rev	GGCATGACACGGGACTCTCC

位点 Locus	基因型 Genotype	样本数 Sample No.	基因型频率 Genotype frequency	等位基因 Allele	等位基因频率 Allele frequency	He	Ho	Ne	PIC
FITM2	CC	640	0.57	C	0.74	0.38	0.62	1.61	0.31
g.72704027	CT	391	0.34
C>T	TT	97	0.09	T	0.26

位点 Locus	基因型 Genotype	样本数 Sample No.	基因型频率 Genotype frequency	等位基因 Allele	等位基因频率 Allele frequency	He	Ho	Ne	PIC
FITM2	CC	640	0.57	C	0.74	0.38	0.62	1.61	0.31
g.72704027	CT	391	0.34
C>T	TT	97	0.09	T	0.26

性状 Traits	日龄 Day	基因型 Genotype
性状 Traits	日龄 Day	CC	CT	TT
个体数 No.		635	389	97
体质量/kg	80	18.652±4.407a	18.422±4.297a	18.030±4.321a
Body weight	100	23.251±5.651a	23.017±5.523ab	22.347±5.434b
	120	28.515±6.656a	28.425±6.447ab	27.370±6.658b
	140	34.064±7.385a	34.084±7.037a	32.606±7.425b
	160	39.596±8.132a	39.438±7.664a	38.368±7.866a
	180	44.302±8.437a	44.167±7.978a	43.070±7.985a
体高/cm	80	53.532±2.994a	53.370±3.153a	53.521±3.223a
Body height	100	56.203±3.285a	56.147±3.319a	56.041±3.284a
	120	58.973±3.363a	58.869±3.184a	58.546±3.189a
	140	61.753±3.298a	61.618±3.303a	60.840±3.110b
	160	63.876±3.278a	63.855±3.162ab	63.191±3.289b
	180	65.372±3.280a	65.406±3.256a	65.103±3.112a
体长/cm	80	57.009±4.096a	56.405±4.095b	56.619±4.233ab
Body length	100	60.015±4.362a	59.680±4.129a	59.747±4.849a
	120	63.609±4.302a	63.433±4.167a	62.608±4.609b
	140	67.380±4.265a	67.221±4.312a	66.052±4.498b
	160	70.375±4.283a	70.201±4.250ab	69.376±4.077b
	180	72.429±4.298a	72.563±4.299a	71.809±4.320a
胸围/cm	80	59.509±4.853a	59.412±4.889a	59.072±5.473a
Chest circumference	100	63.739±4.904a	63.661±4.866ab	62.830±4.665b
	120	67.609±5.037ab	67.917±4.856a	66.820±5.278b
	140	72.577±5.334a	72.902±5.162a	72.113±5.488a
	160	77.357±5.635a	77.584±5.278a	76.856±5.893a
	180	81.689±5.965a	81.528±5.595a	80.943±5.700a
管围/cm	80	6.467±0.592a	6.460±0.581a	6.451±0.576a
Cannon circumference	100	6.858±0.593a	6.839±0.595a	6.759±0.505a
	120	7.334±0.657a	7.335±0.634a	7.253±0.716a
	140	7.634±0.704a	7.640±0.661a	7.530±0.718a
	160	7.950±0.685a	7.933±0.636a	7.869±0.674a
	180	8.193±0.629a	8.185±0.620a	8.113±0.640a

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