Effects of Nano-selenium on the Transcriptome of Sugar Beet Leaves

doi:10.7668/hbnxb.20194488

Abstract

Abstract:

In order to reveal the molecular mechanism of the response to exogenous selenium of sugar beet,seeds of the sugar beet line HD802 were used as test materials for hydroponic experiments.The concentrations of nano-selenium were set to 0(water spray,control),20,50,80,100,150,200 mg/L,respectively.When the plants grew to 8 true leaves stage,nano-selenium solution was evenly sprayed.After 24 h,the activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT)and the content of malondialdehyde(MDA)were measured.After data analysis,50,150 mg/L nano-selenium treated leaves were selected for examination.RNA-Seq was performed to analyze the differentially expressed genes and significant enrichment pathways.The results showed that 50 mg/L nano-selenium treatment had promoting effects on the growth of sugar beet leaves,while 150 mg/L treatment had destructive effects on sugar beet leaves.A total of 9 161 DEGs were identified,of which 3 717 were up-regulated and 5 444 were down-regulated.GO functional enrichment was mainly enriched in processes such as signal transduction,cell communication, integral component of membrane, intrinsic component of membrane, primary metabolic process, organic substance metabolic process, and biological process. The metabolic pathways of KEGG mainly included plant pathogen interaction,plant hormone signal transduction,and other pathways.Transcription factor analysis involved 11 families,including AP2,zf-Dof,HLH,WRKY,HSF_DNA-bind,NAM,zf-BED and Homeobox.It found the optimal concentration for selenium treatment to promote the growth and development of sugar beet seedlings,and preliminarily screened the relevant genes responsive to exogenous selenium in sugar beet.

Key words: Sugar beet, Nano-selenium, RNA-Seq, Differential gene, Metabolic pathway

ZHENG Wenzhe, WANG Yingying, ZHANG Hui, ZHANG Bizhou, SUN Mengyuan, WANG Liang, ZHANG Huizhong, LI Xiaodong, FU Zengjuan, ZHAO Shangmin, E Yuanyuan, ZHANG Ziqiang. Effects of Nano-selenium on the Transcriptome of Sugar Beet Leaves[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(3): 51-59. doi: 10.7668/hbnxb.20194488.

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[1]	陈长兰, 郇丰宁, 孟雪莲, 吕晶. 硒对人体的作用机理及科学补硒方法[J]. 辽宁大学学报(自然科学版), 2016, 43(2):155-168,92.doi:10.16197/j.cnki.lnunse.2016.02.011.
	Chen C L, Huan F N, Meng X L, Lü J. The effects of selenium on human health and diseases and the scientifically supplementation of selenium to human bodies[J]. Journal of Liaoning University(Natural Sciences Edition), 2016, 43(2):155-168,92.
[2]	刘超, 王晋民, 魏廷珍, 韦梅琴, 杨春江, 熊辉岩. 青海乐都富硒区6种主要蔬菜富硒能力研究[J]. 西北农林科技大学学报(自然科学版), 2014, 42(5):157-161.doi:10.13207/j.cnki.jnwafu.2014.05.016.
	Liu C, Wang J M, Wei T Z, Wei M Q, Yang C J, Xiong H Y. Selenium enrichment capacities of six major vegetables in selenium-rich region of Ledou,Qinghai Province[J]. Journal of Northwest A&F University(Natural Science Edition), 2014, 42(5):157-161.
[3]	郭文慧, 刘庆, 史衍玺. 紫甘薯对硒的吸收和累积特征[J]. 中国土壤与肥料, 2016(4):133-138.doi:10.11838/sfsc.20160423.
	Guo W H, Liu Q, Shi Y X. The characteristics of selenium absorption and accumulation in purple sweet potato[J]. Soil and Fertilizer Sciences in China, 2016(4):133-138.
[4]	赵少华, 宇万太, 张璐, 沈善敏, 马强. 环境中硒的生物地球化学循环和营养调控及分异成因[J]. 生态学杂志, 2005, 24(10):1197-1203.
	Zhao S H, Yu W T, Zhang L, Shen S M, Ma Q. Biogeochemical cycling of selenium,nutrition adjustment and differentiation cause in environment[J]. Chinese Journal of Ecology, 2005, 24(10):1197-1203.
[5]	王佳琦, 张子萱, 刘乃新. 外源硒处理条件下红甜菜苗期矿物质积累特性分析[J]. 中国农学通报, 2022, 38(32):1-5.doi:10.11924/j.issn.1000-6850.casb2021-1172.
	Wang J Q, Zhang Z X, Liu N X. Mineral accumulation characteristics of red beet seedlings under exogenous selenium treatment[J]. Chinese Agricultural Science Bulletin, 2022, 38(32):1-5. doi: 10.11924/j.issn.1000-6850.casb2021-1172
[6]	覃广泉, 陈平. 硒对水稻幼苗生长及磷分布效应的影响[J]. 热带农业科学, 2004, 24(5):31-33,92.doi:10.3969/j.issn.1009-2196.2004.05.008.
	Qin G Q, Chen P. Effects of selenium on growth of rice seedlings and ³²P distribution[J]. Chinese Journal of Tropical Agriculture, 2004, 24(5):31-33,92.
[7]	马志军, 郭春景, 程国利. 硒及富硒腐植酸生物液体肥对主要粮食作物应用效果的研究[J]. 腐植酸, 2004(4):23-28, 33.doi:10.19451/j.cnki.issn1671-9212.2004.04.008.
	Ma Z J, Guo C J, Cheng G L. Study on the application effect of selenium and selenium-enriched humic acid biological liquid fertilizer on main grain crops[J]. Humic Acid, 2004(4):23-28,33.
[8]	池忠志, 郑家国, 姜心禄, 杨洋, 杨福明. 硒肥喷施时期对水稻产量和籽粒中硒含量的影响[J]. 耕作与栽培, 2010(4):13-14.doi:10.13605/j.cnki.52-1065/s.2010.04.012.
	Chi Z Z, Zheng J G, Jiang X L, Yang Y, Yang F M. Effect of spraying time of selenium fertilizer on rice yield and selenium content in grain[J]. Tillage and Cultivation, 2010(4):13-14.
[9]	魏廷珍. 青海省平安县富硒区几类蔬菜硒吸收及转化研究[D]. 西宁: 青海大学, 2014.
	Wei T Z. Study on selenium absorption and transformation of several vegetables in Ping'an Se-rich Region of Qinghai Province[D]. Xining: Qinghai University, 2014.
[10]	尚庆茂, 李平兰. 硒在高等植物中的生理作用[J]. 植物生理学通讯, 1998, 34(4):284-288.doi:10.13592/j.cnki.ppj.1998.04.022.
	Shang Q M, Li P L. Physiological action of selenium in higher plants[J]. Plant Physiology Communications, 1998, 34(4):284-288.
[11]	果秀敏, 牛君仿, 方正, 纪姝晶. 植物中硒的形态及其生理作用[J]. 河北农业大学学报, 2003, 26(S1):142-143,147.doi:10.3969/j.issn.1000-1573.2003.z1.043.
	Guo X M, Niu J F, Fang Z, Ji S J. On the forms of selenium in plants and mechanisms of physiological role of selenium[J]. Journal of Hebei Agricultural University, 2003, 26(S1):142-143,147.
[12]	王晶英. 植物生理生化实验技术与原理[M]. 哈尔滨: 东北林业大学出版社, 2003.
	Wang J Y. Experimental techniques and principles of plant physiology and biochemistry[M]. Harbin: Northeast Forestry University Press, 2003.
[13]	李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000.
	Li H S. Principles and techniques of plant physiology and biochemistry experiments[M]. Beijing: Higher Education Press, 2000.
[14]	中国科学院上海植物生理研究所, 上海市植物生理学会. 现代植物生理学实验指南[M]. 北京: 科学出版社, 1999.
	Shanghai Institute of Plant Physiology,Chinese Academy of Sciences, Shanghai Society of Plant Physiology. Guidelines for modern plant physiology experiments[M]. Beijing: Science Press, 1999.
[15]	袁伟玲, 刘志雄, 吴金平, 殷红清, 陈磊夫. 硒对生菜生长、品质、养分吸收和硒转化率的影响[J]. 华北农学报, 2020, 35(S1):189-194.doi:10.7668/hbnxb.20191595.
	Yuan W L, Liu Z X, Wu J P, Yin H Q, Chen L F. Effects of exogenous selenium on the mineral element absorption of lettuce[J]. Acta Agriculturae Boreali-Sinica, 2020, 35(S1):189-194.
[16]	孙崇庆, 马晓春, 高艳明, 李建设. 硒肥对植物工厂水培生菜生长及品质的影响[J]. 中国瓜菜, 2020, 33(6):24-29.doi:10.16861/j.cnki.zggc.2020.0132.
	Sun C Q, Ma X C, Gao Y M, Li J S. Effect of selenium fertilizers on growth and quality of hydroponic lettuce in plant factory[J]. China Cucurbits and Vegetables, 2020, 33(6):24-29.
[17]	胡万行, 石玉, 程玉琦, 赵博思, 周云云, 张毅. 纳米硒对紫色马铃薯生长及其矿质元素含量和品质特性的影响[J]. 西北植物学报, 2020, 40(2):296-303.doi:10.7606/j.issn.1000-4025.2020.02.0296.
	Hu W X, Shi Y, Cheng Y Q, Zhao B S, Zhou Y Y, Zhang Y. Effects of nano-selenium on the growth and its mineral element contents and quality characteristics of purple potatoes[J]. Acta Botanica Boreali-Occidentalia Sinica, 2020, 40(2):296-303.
[18]	张承东, 韩朔睽, 魏钟波. 硒对除草剂胁迫下水稻幼苗活性氧清除系统响应的作用[J]. 环境科学, 2002, 23(4):93-96.doi:10.13227/j.hjkx.2002.04.019.
	Zhang C D, Han S K, Wei Z B. Effect of selenium on the response of the active oxygen scavenging system in the leaves of paddy rice under the stress of herbicide[J]. Chinese Journal of Enviromental Science, 2002, 23(4):93-96.
[19]	王建伟, 何晓玲, 崔金霞, 樊新民, 刘慧英. 外源硒对NaCl胁迫下加工番茄幼苗膜脂过氧化和AsA-GSH循环的影响[J]. 新疆农业科学, 2014, 51(10):1814-1820.doi:10.6048/j.issn.1001-4330.2014.10.010.
	Wang J W, He X L, Cui J X, Fan X M, Liu H Y. Effect of exogenous selenium on membrane lipid peroxidation and ascorbate-glutathione cycle of tomato seedlings under salt stress[J]. Xinjiang Agricultural Sciences, 2014, 51(10):1814-1820.
[20]	李波, 王军, 孙思. 植物诱导抗病机制的研究进展[J]. 中国植保导刊, 2013, 33(9):19-24.doi:10.3969/j.issn.1672-6820.2013.09.004.
	Li B, Wang J, Sun S. Research progress on mechanism of plant induced disease resistance[J]. China Plant Protection, 2013, 33(9):19-24.
[21]	Xie J, Wu Y Y, Zhang T Y, Zhang M Y, Zhu W W, Gullen E A, Wang Z J, Cheng Y C, Zhang Y X. New and bioactive natural products from an endophyte of Panax notoginseng[J]. RSC Advances, 2017, 7(60):38100-38109.doi:10.1039/C7RA07060H.
[22]	杨秀娟. 紫花苜蓿抗寒性评价及其对秋冬季节低温适应性[D]. 北京: 北京林业大学, 2006.
	Yang X J. Study on the evaluation of alfalfa(Medicago sativa)cold resistance and physiological response in autumn and winter cold stress[D]. Beijing: Beijing Forestry University, 2006.
[23]	赵阳阳, 许智慧, 刘妍, 韩晋, 徐东平. 细胞色素P450基因多态性与药物代谢研究进展[J]. 临床药物治疗杂志, 2017, 15(4):1-6.doi:10.3969/j.issn.1672-3384.2017.04.001.
	Zhao Y Y, Xu Z H, Liu Y, Han J, Xu D P. Research progress in genetic polymorphism of CYP450 enzymes related to drug metabolism[J]. Clinical Medication Journal, 2017, 15(4):1-6.
[24]	Pinot F, Beisson F. Cytochrome P450 metabolizing fatty acids in plants:characterization and physiological roles[J]. The FEBS Journal, 2011, 278(2):195-205.doi:10.1111/j.1742-4658.2010.07948.x.
[25]	Spector A A, Kim H Y. Cytochrome P450 epoxygenase pathway of polyunsaturated fatty acid metabolism[J]. Biochimica et Biophysica Acta, 2015, 1851(4):356-365.doi:10.1016/j.bbalip.2014.07.020. pmid: 25093613
[26]	McLean K J, Hans M, Munro A W. Cholesterol,an essential molecule:diverse roles involving cytochrome P450 enzymes[J]. Biochemical Society Transactions, 2012, 40(3):587-593.doi:10.1042/BST20120077.
[27]	Tamiru M, Undan J R, Takagi H, Abe A, Yoshida K, Undan J Q, Natsume S, Uemura A, Saitoh H, Matsumura H, Urasaki N, Yokota T, Terauchi R. A cytochrome P450,OsDSS1,is involved in growth and drought stress responses in rice(Oryza sativa L.)[J]. Plant Molecular Biology, 2015, 88(1/2):85-99.doi:10.1007/s11103-015-0310-5.
[28]	Paddon C J, Westfall P J, Pitera D J, Benjamin K, Fisher K, McPhee D, Leavell M D, Tai A, Main A, Eng D, Polichuk D R, Teoh K H, Reed D W, Treynor T, Lenihan J, Jiang H, Fleck M, Bajad S, Dang G, Dengrove D, Diola D, Dorin G, Ellens K W, Fickes S, Galazzo J, Gaucher S P, Geistlinger T, Henry R, Hepp M, Horning T, Iqbal T, Kizer L, Lieu B, Melis D, Moss N, Regentin R, Secrest S, Tsuruta H, Vazquez R, Westblade L F, Xu L, Yu M, Zhang Y, Zhao L, Lievense J, Covello P S, Keasling J D, Reiling K K, Renninger N S, Newman J D. High-level semi-synthetic production of the potent antimalarial artemisinin[J]. Nature, 2013, 496(7446):528-532.doi:10.1038/nature12051.
[29]	Han J Y, Kim M J, Ban Y W, Hwang H S, Choi Y E. The involvement of β-amyrin 28-oxidase(CYP716A52v2)in oleanane-type ginsenoside biosynthesis in Panax ginseng[J]. Plant and Cell Physiology, 2013, 54(12):2034-2046.doi:10.1093/pcp/pct141.
[30]	Han J Y, Kim H J, Kwon Y S, Choi Y E. The cyt P450 enzyme CYP716A47 catalyzes the formation of protopanaxadiol from dammarenediol-Ⅱ during ginsenoside biosynthesis in Panax ginseng[J]. Plant and Cell Physiology, 2011, 52(12):2062-2073.doi:10.1093/pcp/pcr150.
[31]	Han J Y, Hwang H S, Choi S W, Kim H J, Choi Y E. Cytochrome P450 CYP716A53v2 catalyzes the formation of protopanaxatriol from protopanaxadiol during ginsenoside biosynthesis in Panax ginseng[J]. Plant and Cell Physiology, 2012, 53(9):1535-1545.doi:10.1093/pcp/pcs106.
[32]	Höfer R, Boachon B, Renault H, Gavira C, Miesch L, Iglesias J, Ginglinger J F, Allouche L, Miesch M, Grec S, Larbat R, Werck-Reichhart D. Dual function of the cytochrome P450 CYP76 family from Arabidopsis thaliana in the metabolism of monoterpenols and phenylurea herbicides[J]. Plant Physiology, 2014, 166(3):1149-1161.doi:10.1104/pp.114.244814. pmid: 25082892
[33]	王丽玲, 李焰生, 王鹏飞. 抑制低密度脂蛋白受体相关蛋白2表达对Alzheimer's病细胞模型丝裂原活化蛋白激酶信号通路影响的机制研究[J]. 临床神经病学杂志, 2020, 33(1):40-46.doi:10.3969/j.issn.1004-1648.2020.01.011.
	Wang L L, Li Y S, Wang P F. Study on mechanism of inhibiting low-density lipoprotein receptor-related protein 2 expression on mitogen-activated protein kinase signaling pathway in Alzheimer's disease cell models[J]. Journal of Clinical Neurology, 2020, 33(1):40-46.
[34]	Wei G F, Dong L L, Yang J, Zhang L J, Xu J, Yang F, Cheng R Y, Xu R, Chen S L. Integrated metabolomic and transcriptomic analyses revealed the distribution of saponins in Panax notoginseng[J]. Acta Pharmaceutica Sinica B, 2018, 8(3):458-465.doi:10.1016/j.apsb.2017.12.010.
[35]	Xie J, Wu Y Y, Zhang T Y, Zhang M Y, Peng F, Lin B, Zhang Y X. New antimicrobial compounds produced by endophytic Penicillium janthinellum isolated from Panax notoginseng as potential inhibitors of FtsZ[J]. Fitoterapia, 2018, 131:35-43.doi:10.1016/j.fitote.2018.10.006.
[36]	魏海超, 刘媛, 豆明珠, 杨素欣, 冯献忠. 大豆AP2/ERF基因家族的分子进化分析[J]. 植物生理学报, 2015, 51(10):1706-1718.doi:10.13592/j.cnki.ppj.2015.0396.
	Wei H C, Liu Y, Dou M Z, Yang S X, Feng X Z. Molecular evolution of AP2/ERF gene family in Glycine max[J]. Plant Physiology Journal, 2015, 51(10):1706-1718.
[37]	陈琼琼, Abdullah Shalmani, 陈云波, 黄洋彬, 陈坤明, 李文强. 水稻NAM基因家族的全基因组鉴定及表达分析[J]. 西北植物学报, 2020, 40(6):907-917.doi:10.7606/j.issn.1000-4025.2020.06.0907.
	Chen Q Q, Shalmani A, Chen Y B, Huang Y B, Chen K M, Li W Q. Genome-wide identification and expression analysis of NAM gene family in rice[J]. Acta Botanica Boreali-Occidentalia Sinica, 2020, 40(6):907-917.
[38]	Ai L P, Shen A, Gao Z C, Li Z L, Sun Q L, Li Y Y, Luan W J. Reverse genetic analysis of transcription factor OsHox9,a member of homeobox family,in rice[J]. Rice Science, 2014, 21(6):312-317.doi:10.1016/s1672-6308(14)60271-7.

样品 Sample	原始读段/条 Raw reads	有效读段/条 Clean reads	过滤后碱基/Gb Clean bases	错误率/% Error rate	Q30/%
CK-1	51 915 302	51 909 586	7.73	0.04	92.05
CK-2	52 796 436	52 790 504	7.87	0.04	92.83
CK-3	57 862 260	57 856 624	8.64	0.04	92.02
150 mg/L-1	36 209 686	36 206 182	5.39	0.04	91.12
150 mg/L-2	41 834 274	41 830 462	6.23	0.04	92.07
150 mg/L-3	40 405 984	40 402 760	6.02	0.04	89.72
50 mg/L-1	42 093 642	42 088 736	6.27	0.04	93.39
50 mg/L-2	41 264 466	41 260 298	6.14	0.04	92.84
50 mg/L-3	37 532 788	37 528 802	5.59	0.04	91.94

样品 Sample	原始读段/条 Raw reads	有效读段/条 Clean reads	过滤后碱基/Gb Clean bases	错误率/% Error rate	Q30/%
CK-1	51 915 302	51 909 586	7.73	0.04	92.05
CK-2	52 796 436	52 790 504	7.87	0.04	92.83
CK-3	57 862 260	57 856 624	8.64	0.04	92.02
150 mg/L-1	36 209 686	36 206 182	5.39	0.04	91.12
150 mg/L-2	41 834 274	41 830 462	6.23	0.04	92.07
150 mg/L-3	40 405 984	40 402 760	6.02	0.04	89.72
50 mg/L-1	42 093 642	42 088 736	6.27	0.04	93.39
50 mg/L-2	41 264 466	41 260 298	6.14	0.04	92.84
50 mg/L-3	37 532 788	37 528 802	5.59	0.04	91.94

样品 Sample	有效读段/条 Clean reads	所有能比对上的读段/条 (比例/%) Total mapped	唯一比对读段/条 (比例/%) Unique mapped	多比对读段/条(比例/%) Multiple mapped
CK-1	51 909 586	45 368 129(87.40)	40 348 502(77.73)	5 019 627(9.67)
CK-2	52 790 504	46 227 390(87.57)	39 536 828(74.89)	6 690 562(12.67)
CK-3	57 856 624	50 414 842(87.14)	43 496 329(75.18)	6 918 513(11.96)
150 mg/L-1	36 206 182	31 235 013(86.27)	23 271 117(64.27)	7 963 896(22.00)
150 mg/L-2	41 830 462	36 189 429(86.51)	26 865 816(64.23)	9 323 613(22.29)
150 mg/L-3	40 402 760	34 627 031(85.70)	26 259 083(64.99)	8 367 948(20.71)
50mg/L-1	42 088 736	35 996 234(85.52)	25 276 476(60.06)	10 719 758(25.47)
50 mg/L-2	41 260 298	35 137 623(85.16)	27 329 554(66.24)	7 808 069(18.92)
50 mg/L-3	37 528 802	32 247 102(85.93)	25 521 134(68.00)	6 725 968(17.92)

样品 Sample	有效读段/条 Clean reads	所有能比对上的读段/条 (比例/%) Total mapped	唯一比对读段/条 (比例/%) Unique mapped	多比对读段/条(比例/%) Multiple mapped
CK-1	51 909 586	45 368 129(87.40)	40 348 502(77.73)	5 019 627(9.67)
CK-2	52 790 504	46 227 390(87.57)	39 536 828(74.89)	6 690 562(12.67)
CK-3	57 856 624	50 414 842(87.14)	43 496 329(75.18)	6 918 513(11.96)
150 mg/L-1	36 206 182	31 235 013(86.27)	23 271 117(64.27)	7 963 896(22.00)
150 mg/L-2	41 830 462	36 189 429(86.51)	26 865 816(64.23)	9 323 613(22.29)
150 mg/L-3	40 402 760	34 627 031(85.70)	26 259 083(64.99)	8 367 948(20.71)
50mg/L-1	42 088 736	35 996 234(85.52)	25 276 476(60.06)	10 719 758(25.47)
50 mg/L-2	41 260 298	35 137 623(85.16)	27 329 554(66.24)	7 808 069(18.92)
50 mg/L-3	37 528 802	32 247 102(85.93)	25 521 134(68.00)	6 725 968(17.92)

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