Effects of Fertilization on the Stoichiometry of Carbon,Nitrogen and Phosphorus and Enzyme Activities in Agricultural System

doi:10.7668/hbnxb.20192592

Abstract

Abstract:

Stoichiometry characteristics of soil CNP and MBC,MBN,MBP,and the changes of enzyme activity with different fertilization can help to understand the relationship between fertilization and soil nutrient balance,and thus formulate more reasonable fertilization measures.Published literature data on the changes of CNP status and enzyme activity in fertilized soil were collected,and the changes of CNP stoichiometry and enzyme activity in cultivated soil nutrient/biomass with different fertilization were analyzed by Meta-analysis.The results showed that the C/N under single fertilizer,compound fertilizer and the application of chemical fertilizer and manure was 14.1,14.8 and 23.2,the value of C/P was 20.4,15.3 and 14.8,the value of N/P was 5.03,4.43 and 3.10,respectively.There was no significant difference in MBC/MBN and MBN/MBP with different fertilization.The ratio of MBC/MBP was:application of fertilizer and manure>single fertilizer>compound fertilizer.Different fertilizations could improve the activities of soil sucrase,phosphatase,urease and catalase,especially the application of chemical fertilizer and manure(which increased urease activity by 100%).It showed that the application of chemical fertilizer and manure could significantly affected the stoichiometric ratio of CNP in soil,enhanced microbial activities and promoted microorganisms to release more enzymes conducive to soil nutrient mineralization.It was an effective way to improve crop yield and ensure soil health in agricultural production.Soil enzyme activity was restricted by soil fertility and microbial process.A certain correlation between CNP stoichiometric ratio and soil enzyme activity was observed,which was also affected by land use type,soil pH and rotation system.The study of soil nutrient-microorganism-enzyme activity system can provide a theoretical basis for optimizing fertilization management in farmland system.

Key words: Agricultural soil, Fertilization, Meta-analysis, Enzyme activity, CNP stoichiometry

PING Huaixiang, CUI Jianyu, CHEN Shuo, WEI Lulu, CHEN Qing, ZHANG Delong. Effects of Fertilization on the Stoichiometry of Carbon,Nitrogen and Phosphorus and Enzyme Activities in Agricultural System[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 112-120. doi: 10.7668/hbnxb.20192592.

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Figures/Tables 7

Fig.1 C/N,C/P and N/P under different fertilization
Different letters indicate significant difference(P<0.05) of C/N,C/P and C/P with different fertilization.

Fig.2 Weight response ration(RR₊₊)of C/N,C/P and N/P with different fertilization,land use types, soil pH and rotation systems
Numbers in brackets indicate the number of samples.The same as Fig.4,6.

Fig.3 MBC/MBN,MBC/MBP and MBN/MBP with different fertilization
Different letters indicate significant difference(P<0.05)of MBC/MBN,MBC/MBP and MBC/MBP with different fertilization.

Fig.4 Weight response ration(RR₊₊)of MBC/MBN, MBC/MBP and MBN/MBP with different fertilization, land use types,soil pH and rotation systems

Fig.5 Increment of the activities of sucrase,phosphatase, urease and catalase with different fertilization
Different letters indicate significant difference(P<0.05)of the increment of the activities of sucrase,phosphatase,urease and catalase with different fertilization.

Fig.6 Weight response ration(RR₊₊)of sucrase,phosphatase,urease and catalase activities with different fertilization,land use types,soil pH and rotation systems

Tab.1 Correlation between soil nutrients, stoichiometry ratio of microbial biomass, and enzyme activity

指标 Index	有机碳 SOC	全氮 TN	全磷 TP	微生物量碳 MBC	微生物量氮 MBN	微生物量磷 MBP	蔗糖酶 Sucrase	磷酸酶 Phosphatase	脲酶 Urease
全氮TN	0.28^**
全磷TP	0.38^**	0.38^**
微生物量碳MBC	0.37^**	0.46^**	-0.15ns
微生物量氮MBN	0.28^**	0.59^**	0.44^**	0.57^**
微生物量磷MBP	0.60^**	0.85^**	0.72^**	0.22^*	0.72^**
蔗糖酶 Sucrase	0.03ns	-0.12ns	-0.01ns	-0.05ns	-0.27^*	-0.86^**
磷酸酶 Phosphatase	0.34^**	0.03ns	-0.07ns	0.64^**	0.17ns	0.04ns	0.85^**
脲酶 Urease	0.08ns	0.61^**	0.55^**	-0.06ns	-0.27^*	0.44^*	-0.09ns	-0.08ns
过氧化氢酶 Catalase	-0.45^**	0.13ns	0.26ns	0.11ns	0.89^**	0.90^**	0.07ns	-0.06ns	0.17ns

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