Development of a New Set of EST-SSR Markers and Their Application in Genetic Map Construction in Wheat

doi:10.7668/hbnxb.20191046

Abstract

Abstract: In order to enrich the mumber of wheat functional molecular markers,lay the foundation for the research of wheat functional gene location,copmarative genomics,wheat origin and evolution. In previous studies,8 389 potential EST-SSRs were identified from wheat transcriptome data. In the present study,585 pairs of primers were randomly selected for further analysis from 8 389 EST-SSR primers with a primer score of more than 95 points. And a wheat genetic map for the recombinant inbred line population derived from Chuanmai 42×Chuannong 16 was constructed using these newly developed EST-SSR markers and combined with SRAP markers and SSR markers. The results showed that 555 of the 585 pairs of primers were able to amplify the stable and clear bands in Chuanmai 42 and Chuannong 16,and the primer efficacy was 94.87%. Of these,44 pairs of EST-SSRs exhibited obvious and stable polymorphisms in parents with a polymorphism rate of 7.93%. There were 12 linkage groups in the genetic map containing newly developed EST-SSR markers. And these 12 linkage groups were composed of 163 markers,including 17 EST-SSRs markers,73 SSR markers,and 73 SRAP markers.The average distance between adjacent markers of 13.11 cM,and covering wheat genome length of 1 551.5 cM. This study enriched the number of functional molecular markers in wheat and laid the foundation for the study of wheat functional genes localization,comparative genomics,wheat origin and evolution.

Key words: Wheat, EST-SSR marker, Genetic map, RIL

CLC Number:

Q37
S512.03

LV Bingbing, DAI Chang, PENG Zhengsong, YAMAMOTO Naoki, WU Yichao, WEI Shuhong, YANG Zaijun. Development of a New Set of EST-SSR Markers and Their Application in Genetic Map Construction in Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(4): 57-63. doi: 10.7668/hbnxb.20191046.

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