Cloning and Tissue Cell Expression Pattern Analysis of Apolipoprotein E (APOE) Gene in Goat

doi:10.7668/hbnxb.20190578

Abstract

Abstract: The aim of this study was to clone goat apolipoprotein E (APOE) gene sequence, analyze its biological characteristics and elucidate the expression patterns of APOE in goat different tissues and differentiated stages of preadipocytes. The goat APOE gene sequence was cloned by RT-PCR and 3'RACE technique. The biological characteristics of goat APOE were analyzed by a variety of online tools. In addition, the expression levels of goat APOE in different tissues and differentiated stages of intramuscular adipocytes were investigated by qPCR technique. The obtained length of goat APOE sequence was 970 bp, including 951 bp ORF, 7 bp 5'UTR and 12 bp 3'UTR (GenBank accession number:MN049956); 152 bp 3'UTR cloned by 3' RACE technique(Accession number:MN049957). Targetscan and mirbase predicted that miR-22-3p may target the goat APOE. The goat APOE encoded 316 amino acids and formed an unstable acidic protein with SignalIP 4.1 peptide without transmembrane domain. Subcellular localization results showed that APOE playing biological role in extracellular, cytoplasm, vacuole, nucleus and endoplasmic reticulum. The phylogenetic tree showed that the homology of APOE was close to Ovis aries, Pantholops hodgsonii and Bos mutus. Tissue expression profile revealed that goat APOE gene had highest expression level in subcutaneous fat, which was significantly higher than other analyzed tissues (P<0.01). Meanwhile, time-series expression profile in subcutaneous preadipocytes indicated that the highest expression level of APOE reached at 60 h. This study provides basic theoretical data for further revealing the role of APOE gene in adipocyte differentiation, fat deposition and lipid metabolism.

Key words: Goat, APOE, Cloning, Tissue expression, Temporal expression

CLC Number:

Q78
S826

DU Yu, WANG Yong, ZHANG Yanan, XU Qing, ZHU Jiangjiang, LIN Yaqiu. Cloning and Tissue Cell Expression Pattern Analysis of Apolipoprotein E (APOE) Gene in Goat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(3): 217-224. doi: 10.7668/hbnxb.20190578.

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