Cloning and Expression Analysis of a Cotton Pigment Gland Formation Related Gene GhNAC201

doi:10.7668/hbnxb.20190587

Abstract

Abstract: To investigate the function of GhNAC201 in cotton pigment gland development,RT-PCR was performed to isolate the coding sequence(CDS)of GhNAC201 from glanded cotton Zhongmiansuo 12. Gene expressions of glanded and glandless cotton at different developmental stages,in different tissues and under various hormones treatment were analyzed by qRT-PCR. CDS of GhNAC201 was 948 bp in length and encoded 315 aa,with a theoretical pI 5.97. 27.94% alpha helix,5.40% beta turn,18.10% extended strand and 48.57% random coil were predicted in protein secondary structure. 3D structure of GhNAC201 had been modelled by rice stress-responsive NAC1 with model dimensions of X:55.229 Å,Y:36.150 Å,Z:46.112 Å. GhNAC201 contained a NAM domain at 30-163 aa,and 5 threonine,6 serine and 4 tyrosine phosphorylation sites were predicted within the NAM domain. Subcellular localization prediction showed that GhNAC201 had a certain probability to locate in secretory pathway or other subcellular structures other than mitochondrion and chloroplast. The homology of GhNAC201 homologous proteins in different species was relatively high,and 5 motifs were highly conserved.The motif pattern of GhNAC201 was consistent with its homologous proteins in Gossypium arboretum and G. raimondii. Expression levels of GhNAC201 in the glanded cotton Zhongmiansuo 12 were extremely significantly higher than those in dominant and recessive glandless cotton and could be extremely significantly up-regulated by ABA,BR,GA and MeJA. The spatial-temporal expression pattern of GhNAC201 in Zhongmiansuo 12 tended to be consistent with the spatial-temporal distribution of gland density. In conclusion, GhNAC201 was closely related to the development of pigment glands in cotton,and might regulate gland development or gossypol metabolism through ABA,BR,GA and JA pathways.

Key words: Cotton, NAC transcriptional factor, GhNAC201, Sequence analysis, Expression analysis

CLC Number:

S562
Q78

ZHANG Xi, QIAN Yuyuan, LIU Yi, WANG Guang, SONG Shijia, LI Xiaofei, MI Huanfang, CUI Shufang, LI Junlan. Cloning and Expression Analysis of a Cotton Pigment Gland Formation Related Gene GhNAC201[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(1): 29-35. doi: 10.7668/hbnxb.20190587.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.20190587

http://www.hbnxb.net/EN/Y2020/V35/I1/29

References

[1] Cheng H L,Lu C R,Yu J Z,Zou C S,Zhang Y P,Wang Q L,Huang J,Feng X X,Jiang P F,Yang W C,Song G L. Fine mapping and candidate gene analysis of the dominant glandless gene Gl_e² in cotton(Gossypium spp.)[J]. Theoretical and Applied Genetics,2016,129(7):1347-1355.doi:10.1007/s00122-016-2707-1.
[2] Ma D,Hu Y,Yang C Q,Liu B L,Fang L,Wan Q,Liang W H,Mei G F,Wang L J,Wang H P,Ding L Y,Dong C G,Pan M Q,Chen J D,Wang S,Chen S Q,Cai C P,Zhu X F,Guan X Y,Zhou B L,Zhu S J,Wang J W,Guo W Z,Chen X Y,Zhang T Z. Genetic basis for glandular trichome formation in cotton[J]. Nature Communications,2016,22(7):10456.doi:10.1038/ncomms10456.
[3] Zhang W J,Xu Z R,Pan X L,Yan X H,Wang Y B. Advances in gossypol toxicity and processing effects of whole cottonseed in dairy cows feeding[J]. Livestock Science,2007,111(1-2):1-9.doi:10.1016/j.livsci.2007.03.006.
[4] 刘祎,钱玉源,崔淑芳,金卫平,王广恩,张曦,张海娜,李俊兰.低酚棉种子利用研究进展[J].中国棉花,2018,45(8):4-8.doi:10.11963/1000-632X.lyljl.20180828.
Liu Y,Qian Y Y,Cui S F,Jin W P,Wang G E,Zhang X,Zhang H N,Li J L. Research progress on seed utilization of glandless cotton[J]. China Cotton,2018,45(8):4-8.
[5] 刘慧,季灵艳,赵天伦,陈进红,祝水金.陆地棉色素腺体与不同棉酚旋光体含量之间的相关性研究[J].棉花学报,2017,29(5):437-446.doi:10.11963/1002-7807.
lhzsj.20170818.
Liu H,Ji L Y,Zhao T L,Chen J H,Zhu S J. Analysis of the correlation between pigment glands and(-)/(+)-gossypol contents in upland cotton[J]. Cotton Science,2017,29(5):437-446.
[6] 钱玉源,张海娜,崔淑芳,刘祎,王广恩,金卫平,韩轩,李俊兰.棉花色素腺体的研究进展[J].作物研究,2017,31(2):205-210.doi:10.16848/j.cnki.issn.1001-5280.2017.02.24.
Qian Y Y,Zhang H N,Cui S F,Liu Y,Wang G E,Jin W P,Han X,Li J L.Research progress of cotton pigment gland[J]. Crop Research,2017,31(2):205-210.
[7] 胡佳惠,赵天伦,李诚,余静文,陈进红,祝水金. 比克氏棉色素腺体形态建成与棉酚代谢特性的研究[J].棉花学报,2019,31(1):54-63.doi:10.11963/1002-7807.
hjhzsj.20181226.
Hu J H,Zhao T L,Li C,Yu J W,Chen J H,Zhu S J. Morphogenesis of pigment glands and metabolic characteristics of gossypol in Gossypium bickii[J]. Cotton Science,2019,31(1):54-63.
[8] Janga M R,Pandeya D,Campbell L M,Konganti K,Villafuerte S T,Puckhaber L,Pepper A,Stipanovic R D,Schefler J A,Rathore K S. Genes regulating gland development in the cotton plant[J]. Plant Biotechnology Journal,2019,17(6):1142-1153.doi:10.1111/pbi.13044.
[9] Xu Y H,Wang J W,Wang S,Wang J Y,Chen X Y. Characterization of GaWRKY1,a cotton transcription factor that regulates the sesquiterpene synthase gene(+)-δ-cadinene synthase-A [J]. Plant Physiology,2004,135(1):507-515.doi:10.1104/pp.104.038612.
[10] Sun Q,Cai Y F,Zhu X Y,He X H,Jiang H Z,He G H. Molecular cloning and expression analysis of a new WD40 repeat protein gene in upland cotton[J]. Biologia,2012,67(6):1112-1118.doi:10.2478/s11756-012-0103-0.
[11] Shao H B,Wang H Y,Tang X L. NAC transcription factors in plant multiple abiotic stress responses:progress and prospects[J]. Frontiers in Plant Science,2015,6:902.doi:10.3389/fpls.2015.00902.
[12] Shahnejat-Bushehri S,Allu A D,Mehterov N,Thirumalaikumar V P,Alseekh S,Fernie A R,Mueller-Roeber B,Balazadeh S. Arabidopsis NAC transcription factor JUNGBRUNNEN1 exerts conserved control over gibberellin and brassinosteroid metabolism and signaling genes in tomato[J]. Frontiers in Plant Science,2017,8:214.doi:10.3389/fpls.2017.00214.
[13] Sun H,Hu M L,Li J Y,Chen L,Li M,Zhang S Q,Zhang X L,Yang X Y. Comprehensive analysis of NAC transcription factors uncovers their roles during fiber development and stress response in cotton[J]. BMC Plant Biology,2018,18(1):150.doi:10.1186/s12870-018-1367-5.
[14] Li W,Huang G Q,Zhou W,Xia X C,Li D D,Li X B. A cotton(Gossypium hirsutum)gene encoding a NAC transcription factor is involved in negative regulation of plant xylem development[J]. Plant Physiology and Biochemistry,2014,83:134-141.doi:10.1016/j.plaphy.2014.07.022.
[15] Zhao F L,Ma J H,Li L B,Fan S L,Guo Y N,Song M Z,Wei H L,Pang C Y,Yu S X. GhNAC12,a neutral candidate gene,leads to early aging in cotton(Gossypium hirsutum L.)[J]. Gene,2016,576(1):268-274.doi:10.1016/j.gene.2015.10.042.
[16] Zhang J,Huang G Q,Zou D,Yan J Q,Li Y,Hu S,Li X B. The cotton(Gossypium hirsutum)NAC transcription factor(FSN1)as a positive regulator participates in controlling secondary cell wall biosynthesis and modification of fibers[J]. New Phytologist,2017,217(2):625-640.doi:10.1111/nph.14864.
[17] Liu G Z,Li X L,Jin S X,Liu X Y,Zhu L F,Nie Y C,Zhang X L. Overexpression of rice NAC gene SNAC1 improves drought and salt tolerance by enhancing root development and reducing transpiration rate in transgenic cotton[J]. PLoS One,2014,9(1):e86895.doi:10.1371/journal.pone.0086895.777.
[18] Wang W N,Yuan Y L,Yang C,Geng S P,Sun Q,Long L,Cai C W,Chu Z Y,Liu X,Wang G H,Du X M,Miao C,Zhang X,Cai Y F. Characterization,expression,and functional analysis of a novel NAC gene associated with resistance to Verticillium wilt and abiotic stress in cotton[J]. Genes Genomes Genetics,2016,6(12):3951-3961.doi:10.1534/g3.116.034512.
[19] Gunapati S,Naresh R,Ranjan S,Nigam D,Hans A,Verma P C,Gadre R,Pathre U V,Sane A P,Sane V A. Expression of GhNAC2 from G. herbaceum,improves root growth and imparts tolerance to drought in transgenic cotton and Arabidopsis[J]. Science Report,2016,6:24978.doi:10.1038/srep24978.
[20] Guo Y N,Pang C Y,Jia X Y,Ma Q F,Dou L L,Zhao F L,Gu L J,Wei H L,Wang H T,Fan S L,Su J J,Yu S X. An NAM domain gene, GhNAC79,improves resistance to drought stress in upland cotton[J]. Frontiers in Plant Science,2017,8:1657.doi:10.3389/fpls.2017.01657.
[21] He X,Zhu L F,Xu L,Guo W F,Zhang X L. GhATAF1,a NAC transcription factor,confers abiotic and biotic stress responses by regulating phytohormonal signaling networks[J]. Plant Cell Reports,2016,35(10):2167-2179.doi:10.1007/s00299-016-2027-6.
[22] 张曦,钱玉源,刘祎,崔淑芳,金卫平,王广恩,张海娜,李俊兰.棉花色素腺体形成相关NAC转录因子的功能分析[C]//中国农学会棉花分会2018年年会论文汇编.安阳:中国棉花杂志社,2018:130.
Zhang X,Qian Y Y,Liu Y,Cui S F,Jin W P,Wang G E,Zhang H N,Li J L. Functional analysis of NAC transcription factors associated with cotton pigment glandular formation[C]//2018 annual conference of China Society of Cotton Sci-tech. Anyang:Chian Cotton Periodical Office,2018:130.
[23] Zhang X,Zhen J B,Li Z H,Kang D M,Yang Y M,Kong J,Hua J P. Expression profile of early responsive genes under salt stress in upland cotton(Gossypium hirsutum L.)[J]. Plant Molecular Biology Reporter,2011,29(3):626-637.doi:10.1007/s11105-010-0269-y.
[24] 王全喜,张小平.植物学[M].2版.北京:科学出版社,2012:33.
Wang Q X,Zhang X P. Botany[M].2nd ed.Beijing:Science China Press,2012:33.
[25] Benedict C R,Martin G S,Liu J G,Puckhaber L,Magill C W. Terpenoid aldehyde formation and lysigenous gland storage sites in cotton:variant with mature glands but suppressed levels of terpenoid aldehydes[J]. Phytochemistry,2004,65(10):1351-1359.doi:10.1016/j.phytochem.2004.03.032.
[26] Tao T,Zhao L,Lü Y D,Chen J D,Hu Y,Zhang T Z,Zhou B L. Transcriptome sequencing and differential gene expression analysis of delayed gland morphogenesis in Gossypium australe during seed germination[J]. PLoS One,2013,8(9):e75323.doi:10.1371/journal.pone.0075323.
[27] 姜鹏飞,陆才瑞,邹长松,程海亮,杨文翠,冯晓旭,张友平,王巧连,宋国立.棉花无腺体近等基因系差异表达基因分析[J].棉花学报,2015,27(6):506-514.doi:10.11963/issn.1002-7807.201506002.
Jiang P F,Lu C R,Zou C S,Cheng H L,Yang W C,Feng X X,Zhang Y P,Wang Q L,Song G L.Analysis of differentially expressed genes from glandless near-isogenic lines of cotton[J]. Cotton Science,2015,27(6):506-514.
[28] Saga H,Ogawa T,Kai K,Suzuki H,Ogata Y,Sakurai N,Shibata D,Ohta D. Identification and characterization of ANAC042,a transcription factor family gene involved in the regulation of camalexin biosynthesis in Arabidopsis [J]. Molecular Plant-Microbe Interactions,2012,25(5):684.doi:10.1094/MPMI-09-11-0244.
[29] Tian X,Ruan J X,Huang J Q,Fang X,Mao Y B,Wang L J,Chen X Y,Yang C Q. Gossypol:phytoalexin of cotton[J]. Science China Life Sciences,2016,59(2):122-129.doi:10.1007/s11427-016-5003-z.
[30] Shahnejat-Bushehri S,Tarkowska D,Sakuraba Y,Balazadeh S. Arabidopsis NAC transcription factor JUB1 regulates GA/BR metabolism and signaling[J]. Nature Plants,2016,2(3):16013.doi:10.1038/nplants.2016.13.
[31] Jensen M K,Skriver K. NAC transcription factor gene regulatory and protein-protein interaction networks in plant stress responses and senescence[J]. IUBMB Life,2014,66(3):156-166.doi:10.1002/iub.1256.

Please choose a citation manager

Content to export