Genome-wide Characterization and Expression Analysis of Soybean GmGLRs Gene

doi:10.7668/hbnxb.20190234

Abstract

Abstract: The purpose of this study was to illustrate the structure traits of GmGLRs family in soybean and its expression pattern in different tissues. Based on the bioinformatics methods, the genes of GLRs family from Glycine max were identified, and then the gene chromosomal location, phylogenetic relationship, gene structure, transmembrane domain, as well as tissue expression pattern, were systematically analyzed. The results showed that a total of 17 GmGLRs genes were systematically identified from Glycine max var. Williams 82. Chromosome mapping analysis indicated that these genes were distributed on 10 different chromosomes unevenly. There were 5, 3 and 2 chromosomes with 1, 2 and 3 GLRs genes, respectively. These 17 GmGLRs genes were classified into 2 subfamilies within 2 GmGLRs genes in one subfamily and the other 15 GmGLRs genes in another subfamily according to phylogenetic relationship. On the phylogenetic relationship, these genes appeared in pair and showed highly homologous. All of the GmGLRs genes had 6 extrons, except one with 7 extrons. Otherwise, the longest, the shortest and the average length of CDS sequence of GmGLRs genes were 2 844, 2 409 and 2 748 bp respectively, indicating that the length of CDS sequence among these genes was less different. The prediction of transmembrane domain showed that 10, 4, 2 and 1 GmGLRs had 3, 4, 5 and 2 transmembrane domains, respectively. The expression patterns of GmGLRs genes in different tissues showed no spatial differences, but there were significant differences in expression abundance. The number of high, medium and low abundance expressed genes was 8, 5 and 4, respectively. The results of this paper would provide a theoretical basis for cloning and functional research of GmGLRs genes.

Key words: Glycine max, Genome of soybean, GmGLRs gene, Bioinformatics, Expression analysis

CLC Number:

S562.03
Q78

CUI Xiaoxia, ZHAO Shuo, GUO Shuqiao, SHU Hongmei, HE Xiaolan, NI Wanchao, GONG Yuanyong, LIU Laihua. Genome-wide Characterization and Expression Analysis of Soybean GmGLRs Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(6): 1-8. doi: 10.7668/hbnxb.20190234.

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