Cloning of Goat FGF9 Gene and Its Expression Pattern in Myoblast Differentiation

doi:10.7668/hbnxb.201751384

Abstract

Abstract: The aim of this study was to clone the goat FGF9 gene sequence and perform bioinformatics analysis, so as to elucidate the tissue expression characteristics of FGF9 and its differential expression during myoblast differentiation. The experimental animal of this study was the Jianzhou Big-eared sheep. The FGF9 gene sequence was cloned by RT-PCR. Real-time quantitative PCR (qRT-PCR) was performed to detect the expression of FGF9 in different tissues of goat and at different stages of myoblast differentiation. The results showed that the sequence of goat FGF9 gene was 818 bp, with an ORF region of 627 bp, encoding 208 amino acids. The nucleotide sequence of the CDS region was 99% homologous to Bostaurus and Ovisaries. The FGF9 protein had a transmembrane domain and a FGF family homology domain, which was an unstable hydrophilic protein. The FGF9 gene could express in all tissues of goats, and the expression level in the kidney was the highest, which was significantly higher than in other tissues (P<0.01). The expression level of FGF9 gene in the 2nd day of induced differentiation was significantly higher than that before differentiation (P<0.05), and reached a very significant level in the 4th day (P<0.01). This study provided the important data for further exploring the role of FGF9 gene in goat muscle growth.

Key words: Goat, FGF9, Clone, Bioinformatics analysis, Expression characteristics, Myoblast

CLC Number:

S826

HUANG Kai, LIN Yaqiu, ZHU Jiangjiang, MA Jieqiong, WANG Yong. Cloning of Goat FGF9 Gene and Its Expression Pattern in Myoblast Differentiation[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(3): 232-238. doi: 10.7668/hbnxb.201751384.

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