Differential Expression of High Temperature Stress in Anthesis Stage Related Genes of Maize Inbred Lines

doi:10.7668/hbnxb.201750986

Abstract

Abstract: The objective of this study was to investigate the transcriptome differences after and before high temperature stress during maize anthesis stage, and to explore the key genes and proteins which are contributed to the high temperature resistant. The pollen activities of maize inbred lines Chang 7-2 and Zheng 58 were detected by Ampha^TM Z30 pollen activity analyzer (Amphays, Switzerland), firstly. RNA-sequencing, construction library and quality assessment were carried out for two periods before and after high temperature treatment using the Illumina Hiseq2000^TM sequencing platform, then the sequencing results were analyzed by bioinformatics. The differential expression genes (DEGs) were analyzed using edegR software. Key different expression genes and proteins with high temperature resistance were analyzed by VENN mapping, GO functional enrichment, KEGG pathway method and transcription factors analysis.The pollen activity of Chang 7-2 grown in normal condition was 42.89% and the pollen activity which was treated by high temperature during anthesis stage was 24.37%. The pollen activity of Zheng 58 which was grown in normal condition was 64.83% and the pollen activity treated by high temperature was 35.57%. The results showed that 4 176 significant DEGs were detected in Chang 7-2 inbred under normal growth condition and high temperature treatment. 5 487 significant DEGs were detected in Zheng 58 inbred under normal growth condition and high temperature treatment. KEGG pathway enrichment analysis showed that 2 062 differentially expressed genes in Zheng 58 were enriched in 399 related pathways. KEGG pathway enrichment analysis showed that 1 943 DEGs in Chang 7-2 were enriched in 352 related pathways. A total of 55 transcription factor families were obtained by the transcription factor analysis, of which 45 transcriptional factors contained more than 10 differentially expressed genes. In conclusion, Comparing the RNA-sequencing after and before high temperature treatment of Chang 7-2 and Zheng 58, many DEGs were obtained. The differences of response mechanisms between Chang 7-2 and Zheng 58 to anthesis high temperature stress were comparatively analyzed. The three gene families (Hsfs, IAA and PIP₂) and the Hsps proteins are playing significant roles in the regulation of high temperature stress in maize.

Key words: Maize inbred lines, Anthesis stage, High temperature stress, Illumina RNA-sequencing, Differentially expressed genes (DEGs)

CLC Number:

S513.03

LI Chuan, QIAO Jiangfang, ZHU Weihong, DAI Shutao, HUANG Lu, ZHANG Meiwei, LIU Jingbao. Differential Expression of High Temperature Stress in Anthesis Stage Related Genes of Maize Inbred Lines[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(1): 1-11. doi: 10.7668/hbnxb.201750986.

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