Molecular Detection and Evaluation of Disease Resistance Genes of 39 Introduced Wheat Germplasms

doi:10.7668/hbnxb.2018.06.007

Abstract

Abstract: To preliminarily clarify the composition of the resistance genes to stripe rust and powdery mildew in 39 introduced wheat germplasms, co-segregated or tightly-linked markers were used to detect stripe rust resistance genes Yr5, Yr10 and Yr18, and powdery mildew resistance gene Pm4, Pm13 and Pm21. Simultaneously, the disease resistant level of these germplasms were evaluated in the field. The results indicated that there were 6 germplasms carrying Yr18, which showed stable stripe rust resistance and the reaction type was varied from near immune to middle resistance. 21 germplasms were Yr5 linked marker S1320 positive, and 2 ones were Yr10 linked marker SC₂₀₀ positive. These same marker positive germplasms showed differential disease resistant character, which might be associated with the genetic backgrounds of the carrier varieties. In assistant breeding, the detection results of these markers should be further confirmed by inoculation identification. The resistance of Pm4 to powdery mildew was almost lost, for only 1 of the 7 germplasms carrying Pm4 was of disease resistance. In all 39 germplasms, the powdery mildew resistance genes Pm13 and Pm21 could not be detected. Additionally, 2 germplasms Aoayou No.1 and bermude were of both resistance to stripe rust and powdery mildew, which could be used appropriately in disease resistance breeding.The results would be helpful for parental selection and rational utilization of germplasm resources in wheat disease resistance breeding.

Key words: Wheat, Disease resistance genes, Molecular markers, Stripe rust, Powdery mildew

CLC Number:

S512.1

DONG Na, CHEN Xiangdong, HU Tiezhu, LI Gan, ZHANG Yajuan, RU Zhengang. Molecular Detection and Evaluation of Disease Resistance Genes of 39 Introduced Wheat Germplasms[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(6): 49-55. doi: 10.7668/hbnxb.2018.06.007.

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