Abstract: To investigate the difference of bacterial diversity in greenhouse soil and field soil, 14 samples of greenhouse and field soil in middle and Southern Area of Hebei Province were sequenced by using Miseq high-throughput sequencing technology. And the species composition information and the relative abundance information of two sets of samples were obtained from the sequencing result. The results showed that in the greenhouse (M1)and field (N1)soil samples, the composition of the dominant microflora was basically the same. The dominant phylum of all samples were Proteobacteria, Actinobacteria, Acidobacteria, Bacteroidetes, Chloroflexi, Firmicutes and Gemmatimonadetes. The relative abundance of Actinobacteria in the field was more than it in the greenhouse, but the relative abundance of Firmicutes in the field was less than it in the greenhouse. At the class level, more than 15 dominant species were found in all samples, such as Alphaproteobacteria, Acidobacteria, Gammaproteobacteria, Actinobacteria, Deltaproteobacteria, Betaproteobacteria, Gemmatimonadetes, Cytophagia and so on. Among the dominant species, the most abundant relative abundance in greenhouse was Cytophagia, Bacilli and Flavobacteriia, while the relative abundance was significantly reduced by Acidobacteria and β-Proteobacteria. At the genus level, Arthrobacter, Sphingomonas, Devosia, Bacillus, Xanthomonas and Lysobacter were six dominant genera. Arthrobacter was more in the soil of field, while Bacillus, Xanthomonas and Lysobacter were more in the greenhouse. In addition, some of the genera that had nitrogen fixation and degradation and repair capabilities are relatively more abundant in the field. This should be caused by the different planting environments in fields and greenhouses. The research results have certain value for improving arable land, increasing yield and disease resistance.

Key words: High-throughput sequencing, Greenhouse soil, Field soil, Bacterial diversity

CLC Number: 

• S432.4