Subcellular Localization and Expression Analysis of a Soybean Transcription Factor GmWRKY58 in Response to Abiotic Stresses

doi:10.7668/hbnxb.2018.02.008

Abstract

Abstract: WRKY transcription factor plays an important role in plant response to abiotic stress and regulation of resistance gene expression. In order to reveal the role of a soybean transcription factor GmWRKY58 in response to abiotic stresses.The full length of GmWRKY58 cDNA was cloned from soybean,and the amino acid sequence,physiological and biochemical characteristics and evolutionary relationship were deduced. Subcellular localization and the changes of gene expression under different tissues and abiotic stress were studied. The results showed that GmWRKY58 had a 954 bp length open reading frame and encoded a total of 317 amino acids. Subcellular localization results showed that GFP signals combined GmWRKY58 was only detected in the nucleus. Real-time RT-PCR analysis showed that GmWRKY58 was expressed in soybean roots,stems,leave,flowers and pods,and the expression in each tissue of roots,stems and leave was significantly higher than flowers and pods. In soybean roots, GmWRKY58 gene can be significantly induced by abiotic stress factors such as high salinity,drought,low nitrogen and iron deficiency.Under high salt stress, GmWRKY58 gene expression was increased by more than 187.4 times. Moreover,this gene just slightly induced by other abiotic stresses shch as SA,low temperature,low phosphorus and low potassium. The results indicate that GmWRKY58 plays an important regulatory role in abiotic stress process of salt,drought,low nitrogen and iron deficiency in soybean.

Key words: Soybean, Transcription factor, GmWRKY58, Subcellular localization, Abiotic stresses

CLC Number:

Q78
S565.03

ZHANG Hongmei, LIU Xiaoqing, CHEN Huatao, YUAN Xingxing, CUI Xiaoyan, ZHANG Zhimin, HUANG Zhongwen, CHEN Xin. Subcellular Localization and Expression Analysis of a Soybean Transcription Factor GmWRKY58 in Response to Abiotic Stresses[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(2): 49-57. doi: 10.7668/hbnxb.2018.02.008.

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