Abstract: In order to further excavate the related genes of wheat vernalization,explored the regulation mechanism of wheat vernalization,this study screened and cloned an EST sequence from a high-throughput RNA sequencing analysis comparing the transcriptomes with wheat varieties with different developmental characteristics under vernalization and non-vernalization treatment,and named Trx59. The open reading frame (ORF) of Trx59 gene was 372 bp,ecoding 124 amino acids,and contained one thioredoxin domain structure. Expression analysis was conducted by qRT-PCR and the gene function was verification by using VIGS technique. The result showed that the expression level of Trx59 was gradually up-regulated during vernalizaton process;the expression level and time of Trx59 gene in spring variety LC10 was higher and earlier than that in winter variety J841. BSMV:Trx59 recombinant vector was built and inoculated wheat plants of J841. After 14 days of inoculation,the leaves appeared visible light bleaching phenomenon,and the expression level of Trx59 reduced sharply,indicating that Trx59 gene had been significantly suppressed;the spike differentiation process of plants inoculated by BSMV:Trx59 was later than that of the negative control group,deducing that the Trx59 genes may be related to the wheat developmental characteristics.

Key words: Wheat, Vernalization, Expression analysis, VIGS, Functional verification

CLC Number: 

• Q78

• S512.03