Construction and Virulence Analysis of the ssnA Gene Knock-out Mutant of Streptococcus suis Serotype 2

doi:10.7668/hbnxb.2017.04.001

Abstract

Abstract: Streptococcus suis nuclease A (ssnA) is a recently discovered deoxyribonuclease (DNase),which is one of potential virulence factors of Streptococcus suis serotype 2(SS2).To determine the effects of ssnA on virulence,the ssnA deletion mutant (ΔssnA) was constructed by using the suicide vector pSET4S.The growth and hemolytic activity of the ssnA gene mutant strain had no obvious difference compared with the wild-type strain.The ability of ΔssnA mutant to interact with human laryngeal epithelial cell (Hep-2) was evaluated and it exhibited dramatically decreased ability to adhere to and invade Hep-2 cells.For CD1 mice,the LD₅₀ of the mutation strain was 6.17×10⁸ cfu,whereas the LD₅₀ of wild-type strain was 4.42×10⁷ cfu.This mutation was found to exhibit significant attenuation of virulence when evaluated in CD1 mice,suggesting ssnA plays a critical role in the pathogenesis of SS2,the mechanism of this protein is valuable for further analysis.

Key words: Streptococcus suis 2, ssnA, Gene mutant, Virulence

CLC Number:

Q78
S828

LI Miao, LI Chunling, SONG Shuai, YANG Dongxia, CAI Rujian, LI Yan, JIANG Zhiyong, GOU Hongchao, CHU Pinpin. Construction and Virulence Analysis of the ssnA Gene Knock-out Mutant of Streptococcus suis Serotype 2[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(4): 1-6. doi: 10.7668/hbnxb.2017.04.001.

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