The Divergent Role of Sex Determination Gene TASSELSEED2 in Rice

doi:10.7668/hbnxb.2017.03.002

Abstract

Abstract: The development of floral organs is crucial for the production of crops,in which the formation of unisexual flowers by sex-determination is one of the important theoretic issues.The TASSELSEED2 gene is a JA-synthesis gene which regulates the formation of staminate flower in maize.Previous studies showed that TS2 genes of grasses might adopt new or divergent functions during evolution.In order to get insights into the divergent role of TS2,we compared the protein structure of rice TS2 with maize TS2 by bioinformatic tools.The results showed that OsTS2 encodes a typical SDR protein which contains a conserved GASGIG motif and YTASK motif.The substrate catalyzed by OsTS2 protein was also predicted to be similar with that by maize TS2 protein.We Further examined the expression pattens of OsTS2 and maize TS2 in different tissues.RNA-seq data showed that OsTS2 mainly expressed in the inflorescence of rice.In accord with JA and other hormone regulatory elements found in the promoter of OsTS2,the increased expression of OsTS2 was obviously observed by qPCR in MeJA-treated rice.Whereas,the expression of maize TS2 was mainly detected in 8-leaf stage of maize rather than in inflorescence,and its expression was not affected by exogenous methyl jasmonate.Taken together,these results provide a clue that OsTS2 might recruit a divergent role during evolution.

Key words: Rice, Maize, TS2, Methyl jasmonate

CLC Number:

Q786

GUO Yingying, HOU Zhipeng, ZHONG Ming, CHEN Lijing, MA Hui, ZHANG Li, XU Zhengjin, LI Haoge. The Divergent Role of Sex Determination Gene TASSELSEED2 in Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(3): 7-12. doi: 10.7668/hbnxb.2017.03.002.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2017.03.002

http://www.hbnxb.net/EN/Y2017/V32/I3/7

References

[1] 徐乾坤, 任德勇, 李自壮, 等.水稻小穗颖壳发育的研究进展[J].中国水稻科学,2016,30(1):99-105.
[2] Bortiri E,Hake S.Flowering and determinacy in maize[J].Journal of Experimental Botany,2007,58(5):909-916.
[3] Zhang J,Boualem A,Bendahmane A,et al.Genomics of sex determination[J].Current Opinion in Plant Biology,2014,18(1):110-116.
[4] Li Q,Liu B.Genetic regulation of maize flower development and sex determination[J].Planta,2017,245(1):1-14.
[5] Browse J.Jasmonate:preventing the maize tassel from getting in touch with his feminine side[J].Science Signaling,2009,2(59):pe9.
[6] Delong A,Calderon-Urrea A,Dellaporta S L.Sex determination gene TASSELSEED2 of maize encodes a short-chain alcohol dehydrogenase required for stage-specific floral organ abortion[J].Cell,1993,74(4):757-768.
[7] Acosta I F,Laparra H,Romero S P,et al. tasselseed1 is a lipoxygenase affecting jasmonic acid signaling in sex determination of maize[J].Science,2009,323(5911):262-265.
[8] Simon T,Malcomber,Elizabeth A,et al.Evolution of unisexual flowers in grasses(Poaceae)and the putative sex-determination gene, TASSELSEED2 (TS2)[J].New Phytologist,2006,4(170):885-899.
[9] Lebel-Hardenack S,Ye D,Koutnikova H,et al.Conserved expression of a TASSELSEED2 homolog in the tapetum of the dioecious Silene latifolia and Arabidopsis thaliana[J].The Plant Journal:for Cell and Molecular Biology,1997,12(3):515-526.
[10] 俞竹青, 朱骏, 高菊芳, 等.水稻P0491E01 基因调控花药发育的功能分析(简报)[J].分子细胞生物学报,2006,39(5):467-472.
[11] Cai Q,Yuan Z,Chen M,et al.Jasmonic acid regulates spikelet development in rice[J].Nature Communications,2014,5(5):3476.
[12] Zhang B,Wu S,Zhang Y,et al.A high Temperature-Dependent mitochondrial lipase EXTRA GLUME1 promotes floral phenotypic robustness against temperature fluctuation in rice (Oryza sativa L.)[J].PLOS Genetics,2016,12(7):e1006152.
[13] Kinney M S,Columbus J T,Friar E A.Molecular evolution of the maize sex-determining gene TASSELSEED2 in Bouteloua (Poaceae)[J].Molecular Phylogenetics and Evolution,2003,29(3):519-528.
[14] 朱昀, 王猛, 贾志伟, 等.一种从富含多糖的玉米幼穗中提取RNA的方法[J].植物学通报,2007,24(5):624-628.
[15] Li D,Blakey C A,Dewald C,et al.Evidence for a common sex determination mechanism for pistil abortion in maize and in its wild relative Tripsacum[J].Proceedings of the National Academy of Sciences of the United States of America,1997,94(8):4217-4222.
[16] Calderon-Urrea A C,Dellaporta S L.Cell death and cell protection genes determine the fate of pistils in maize[J].Development,1999,126(3):435-441.
[17] Malcomber S T,Kellogg E A.Evolution of unisexual flowers in grasses (Poaceae) and the putative sex-determination gene, TASSELSEED2(TS2)[J].The New Phytologist,2006,170(4):885-899.
[18] Chen Y,Tan B C.New insight in the Gibberellin biosynthesis and signal transduction[J].Plant Signaling & Behavior,2015,10(5):e1000140.
[19] Song S,Qi T,Huang H,et al.Regulation of stamen development by coordinated actions of jasmonate,auxin,and gibberellin in Arabidopsis[J].Molecular Plant,2013,6(4):1065-1073.
[20] Song S,Qi T,Wasternack C,et al.Jasmonate signaling and crosstalk with gibberellin and ethylene[J].Current Opinion in Plant Biology,2014,21:112-119.

Please choose a citation manager

Content to export