Abstract: To study the relationship between rapeseed oil content and phosphoenolpyruvate carboxylase activity,we constructed the seed-specific ihpRNA expression vector targeting PEPC gene in Brassica napus L..A fragment of 315 bp napin promoter and 246 bp exon fragment of PEPC gene were amplified from the genomic DNA of Xiangyou 15.At the same time,a intron(754 bp) of PEPC gene including 4 bp and 18 bp DNA fragment before and after the splice site respectively was cloned into corresponding enzyme sites.According to the structural principle of expression vector,these fragments were cloned into pBluescript Ⅱ SK(+),respectively. Then we inserted the ihpRNA structure into plant expression vector pBI121,designated pBI121.NP⁺IP^-.Transformed Xiangyou 15 by Agrobacterium tumefaciens containing pBI121.NP⁺IP^-,seven transgenic plants from nine anti-kanamycin plants were confirmed by PCR detection.The oil content of transgenic plants was about 10% higher than that of the controls.

Key words: Brassica napus L., PEPC gene, ihpRNA expression vector, Transgenic rapeseed, Oil content

CLC Number: 

• Q78

• S635.03