Establishment of DNA Fingerprint Identity of Arachis Species with High Oil Content

doi:10.7668/hbnxb.2010.06.012

Abstract

Abstract: Establishment of DNA fingerprint identity(ID)of peanut wild relatives is useful for maintaining and utilizing the resource.In the present study, 20 accessions of peanut wild relatives with high oil content were analyzed by SSR technique to establish their DNA fingerprint identity.Forty-six SSR primer pairs with polymorphism were selected from 252 primers to amplify the genomic DNA.Totally 425 polymorphic bands were produced through the 46 primers.These SSR primers amplified 2 to 21 polymorphic bands with average of 9 bands.Neither single—primer nordouble—primer combinations were able to distinguish all the involved accessions.However primer 2E6 of 46 singleprimers and dowble·primer combination 2E6/PM403 could distinguish the 14 and 18 genetypes of the all 20 accessions respectively.Based on the results of three—primer combination detection, five three·primer combinations were able to identify the all 20 accessions involved, of which 2E6/PM403/10H1A combination was the most efficient.Based on SSR data of the primer combination 2E6/PM403/10H1A, and integrated the accession number and primer name, the DNA fingerprint ID of the 20 Arachis three—wild accessions with high oil content was established.The DNA fingerprint information is useful for peanut breeding and resource storing.

Key words: Wild Arachis species, High oil content, SSR fingerprint

CLC Number:

S565.2

ZHAO Xin-yan, HUANG Li, REN Xiao-ping, JIANG Hui-fang, CHEN Yu-ning. Establishment of DNA Fingerprint Identity of Arachis Species with High Oil Content[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(6): 64-70. doi: 10.7668/hbnxb.2010.06.012.

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http://www.hbnxb.net/EN/Y2010/V25/I6/64

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