Sequence Variation at BoLA-DRB1 * Intron 1-exon 2 in Yak and Cattle

doi:10.7668/hbnxb.2016.03.011

Abstract

Abstract: To accumulate more molecular genetics materials for revealing stress resistance and disease resistance breeding by testing the sequence variation at DRB1 gene and analyzing genetic parameters in detecting regionin in yak and cattle.Gannan yak,Qinghai yak,Tianzhu white yak,Datong yak and Cattle were used in this study.The polymorphism of intron 1 and exon 2 in BoLA-DRB1 gene were analyzed using PCR-single-strand conformational polymorphism.The results showed that 4 SNPs and 1 insertion/deletion mutation were detected in intron 1,and 17 SNPs were detected in exon 2,and they were highly polymorphism;Between these two regions,twenty-one haplotypes and the linkage disequilibrium phenomenon were found,and haplptypes A-A₁,A-B₁,B-A₁ and B-B₁ were most common in yak and cattle.The cluster analysis of DRB1 gene exon 2 showed that yak and other 6 species,cattle and goat were the highest on homology and the phylogenetic distance consistent with their genetic relationship. DRB1 gene intron 1 and exon 2 have high of sequence polymorphism,it might be used as genetic marker in yak and cattle.

Key words: Yak, Cattle, DRB1 gene, Intron 1, Exon 2, Polymorphism

CLC Number:

TIAN Zhili, CHEN Jie, HU Jiang, LUO Yuzhu, LIU Xiu, LI Shaobin, GUO Shuzhen, MU Yongjuan. Sequence Variation at BoLA-DRB1 ^* Intron 1-exon 2 in Yak and Cattle[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(3): 72-79. doi: 10.7668/hbnxb.2016.03.011.

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http://www.hbnxb.net/EN/Y2016/V31/I3/72

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