Cloning of Abscission Zone Development Gene of Gossypiumhirsutum L. GhBOP1 and Preliminary Analysis of Its Function

doi:10.7668/hbnxb.2015.03.003

Abstract

Abstract: In this study, a GhBOP1 involved in abscission zone differentiation was examined in boll-shedding,and could be used as a new candidate gene for increasing cotton yield.Here,a cotton homolog of Arabidopsis BOP gene relating differentiation of abscission zone was isolated by PCR,which encoding protein contains BTB and ANK domains revealed by bioinformatics analysis.The phylogenetic tree analysis of GhBOP1 as an entry showed that the protein sequences from various plants have high identity,exhibiting slow evolution in amino acid changes.The results of qRT-PCR analysis showed that GhBOP1 gene predominantly expressed in abscission zone of petiole although it ubiquitously expressed in all organs,e.g.root,stem,leaf,and flower,etc.The expression level of GhBOP1 gene in abscission zone was 20-fold higher than that in leaf.Virus-induced gene silencing (VIGS) technology was employed to repress GhBOP1 gene expression in this study.Under salt stress,VIGS plants kept leaves normally growing,however,the control plants exhibited leaf shedding.In short,these results primarily suggest that GhBOP1 gene is higher expression in abscission zone,and activated abscission zone formation.The molecular mechanism of this gene regulating differentiation and formation of abscission zone is deserved to further study.

Key words: Cotton, Abscission zone, GhBOP1 gene, Virus inducing gene silence

CLC Number:

CHANG Jing, JIN Xin, PANG Jin-huan, SI Huai-jun, ZHANG Ning, WU Jia-he. Cloning of Abscission Zone Development Gene of Gossypiumhirsutum L. GhBOP1 and Preliminary Analysis of Its Function[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(3): 14-19. doi: 10.7668/hbnxb.2015.03.003.

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