Identification and Characterization of Polymorphic SSR in Cultivated Peanut

doi:10.7668/hbnxb.2015.02.032

Abstract

Abstract: Through screening polymorphic SSRs and analyzing its characteristic, it is anticipated that the identified set of highly informative markers should be very useful to initiate constructing SSR genetic linkage map, QTL research, and molecular assistant breeding studies in cultivated groundnut.A total of 1 523 markers designed by different methods were used to screen for polymorphism in 6 peanut accessions with different botanical types, and the characteristics of polymorphic SSR were analyzed in the aspect of amplified fragment length, repeat motif, and repeat number.The result showed that only 43(2.8%) pairs of markers with 123 polymorphic loci expressed polymorphism.Among the 43 polymorphic SSRs, ARS× ×showed the highest polymorphic rate(6.8%).For the combination examined, Shiyaodou and Silihong were the highest with polymorphic loci of 56, while Qinhuangdaoguangyang and Yinduzhaiye were the lowest with polymorphic loci of 21.Fragment length of 80% polymorphic loci were between 150 bp and 300 bp;GA motif(18.8%) was the most abundant, followed by AG(12.5%)and TC(12.5%);the repeat number of 60% of di-nucleotide and tri-nucleotide motif was over 15.

Key words: Peanut, SSR, Polymorphism, Characteristic

CLC Number:

S565.03

HOU Ming-yu, YANG Xin-lei, GUO Hui-min, MU Guo-jun, LIU Li-feng. Identification and Characterization of Polymorphic SSR in Cultivated Peanut[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(2): 183-190. doi: 10.7668/hbnxb.2015.02.032.

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